+ Models MYCMED-557; No. of Pages 5 Please cite this article in press as: Soares IH, et al. In vitro activity of essential oils extracted from condiments against fluconazole-resistant and -sensitiveQ1 Candida glabrata. Journal De Mycologie Médicale (2015), http://dx.doi.org/10.1016/j.mycmed.2015.06.003 SHORT COMMUNICATION/COURTE COMMUNICATION In vitro activity of essential oils extracted from condiments against fluconazole-resistant and -sensitive Candida glabrata L’activite ´ in vitro des huiles essentielles extraites de condiments contre Candida glabrata re ´ sistant ou sensible au fluconazole I.H. Soares a , É.S. Loreto b , L. Rossato a , D.N. Mario a,c, * , T.P. Venturini a , F. Baldissera a , J.M. Santurio b , S.H. Alves a a Pharmaceutical Sciences Postgraduate Program, Federal University of Santa Maria, Santa Maria, RS, Brazil b Pharmacology Postgraduate Program, Federal University of Santa Maria, Santa Maria, RS, Brazil c Meridional Faculty, Passo Fundo, RS, Brazil Received 28 July 2014; received in revised form 22 June 2015; accepted 25 June 2015 KEYWORDS Candida glabrata; Essential oil; Antifungal activity Summary In the present study, the antifungal activity of essential oils obtained from Origa- num vulgare (oregano), Cinnamomum zeylanicum (cinnamon), Lippia graveolens (Mexican oregano), Thymus vulgaris (thyme), Salvia officinalis (sage), Rosmarinus officinalis (rosemary), Ocimum basilicum (basil) and Zingiber officinale (ginger) were assessed against Candida glabrata isolates. One group contained 30 fluconazole-susceptible C. glabrata isolates, and the second group contained fluconazole-resistant isolates derived from the first group after the in vitro induction of fluconazole-resistance, for a total of 60 tested isolates. The broth microdilution methodology was used. Concentrations of 50 mg/mL, 100 mg/mL, 200 mg/mL, 400 mg/mL, 800 mg/mL, 1600 mg/mL and 3200 mg/mL of the essential oils were used, and the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined. Thyme, sage, rosemary, basil and ginger essential oils showed no antifungal activity at the tested concentrations. Antimicrobial activity less than or equal to 3200 mg/mL was observed for oregano, Mexican oregano and cinnamon essential oils. Both the oregano and Mexican oregano Journal de Mycologie Médicale (2015) xxx, xxx—xxx * Corresponding author. Campus UFSM, Prédio 20, sala 4139, 97105-900 Santa Maria, RS, Brazil. E-mail addresses: isaurahs@hotmail.com (I.H. Soares), erico.loreto@gmail.com (E.S. Loreto), luana.farma@hotmail.com (L. Rossato), debora_alves6@hotmail.com (D.N. Mario), tarcieliventurini@gmail.com (T.P. Venturini), janio.santurio@gmail.com (J.M. Santurio), sydneyalves.ufsm@gmail.com (S.H. Alves). Available online at ScienceDirect www.sciencedirect.com http://dx.doi.org/10.1016/j.mycmed.2015.06.003 1156-5233/# 2015 Elsevier Masson SAS. All rights reserved.