_____________________________________________________________________________________________________ *Corresponding author: E-mail: von73vv22@yahoo.com; J. Adv. Microbiol., vol. 23, no. 3, pp. 41-50, 2023 Journal of Advances in Microbiology Volume 23, Issue 3, Page 41-50, 2023; Article no.JAMB.97737 ISSN: 2456-7116 Isolation and Identification of Microorganisms from Makeup Brushes in Rivers State University Nigeria and Its Environs V. N. Agi a* , O. A. Ollor a , C. A. Azike a and C. V. Onwuasoanya a a Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JAMB/2023/v23i3715 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/97737 Received: 18/01/2023 Accepted: 21/03/2023 Published: 29/03/2023 ABSTRACT Components of makeups and cosmetic supply may serve as source and medium for the growth of microorganisms. A vast majority of females nowadays apply makeups in order to enhance their facial looks. Repetitive use and sharing of the makeup brushes by different customers may become a potential pathway for microbial pathogens spread. The major goal of this study was to isolate and identify microbial contaminants of makeup brushes from students within Rivers State University and its environs. And also determine the antimicrobial sensitivity of the isolated microorganisms to different antimicrobial agents. Eighty (80) samples of makeup brushes were collected from different hostels, personal houses, and beauty salons and were cultured by inoculating them into different culture media as Nutrient, Blood and Sabouraud Dextrose agar to isolate bacteria and fungi respectively. The identification of isolated bacteria was confirmed by using pure culture of the Original Research Article