How to Cite: Al-Hasnawi, H., Albazi, W., & Altaee, R. (2022). Potential effects of Alpha lipoic acid on behavioral alteration and glutamate accumulation during d-gal-induced brain aging in male rats. International Journal of Health Sciences, 6(S6), 7974–7983. https://doi.org/10.53730/ijhs.v6nS6.12191 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 9 April 2022, Manuscript revised: 18 June 2022, Accepted for publication: 27 July 2022 7974 Potential effects of Alpha lipoic acid on behavioral alteration and glutamate accumulation during d-gal-induced brain aging in male rats Haider Al-Hasnawi College of Veterinary Medicine /University of Kerbala * Corresponding author email: haider.a@s.uokerbala.edu.iq Wefak Albazi College of Veterinary Medicine /University of Kerbala Raeed Altaee College of Veterinary Medicine /University of Kerbala Abstract---Background: Alpha lipoic acid has both hydrophilic and hydrophobic characteristics and is abundantly distributed in cellular membranes and the cytoplasm. It is among the top cell- protective antioxidants. Material and methods : The present work investigated the possible therapeutic effects of alpha lipoic acid in a male rat model of brain aging induced by D-galactose. Four equal-sized groups of 40 male rats were randomly assigned: G1, the control group, G2, and G3, which each received daily doses of 200 mg/kg of D-gal for 30 days. Alpha lipoic acid was given orally for 30 days to the G4 D-gal + alpha lipoic acid group at 200 mg/kg bw, IP. daily with 100 mg/kg. thirty days of IP. Glutamate is deposited in the brain, according to research on behavioral alterations and brain glutamate. Indicators of oxidative stress are increased Our Results show that whereas brain glutamate deposition declines in the D-gal model of aging, the Forced Swimming Test (FST) and Morris Water Maze Test considerably rise (MWM). According to the study's findings, D-gal brain damage can be enhanced by intubating 100 mg/kg B.W.IP of ALA to counteract its unfavorable effects. Results: value of tissue glutamate shows a significantly decrease (p≤0.0001) after four weeks in G2 treated group when compared to G1, G3 and G4, also there was no significant (p≥0.0001) change between G1, G3 and G4 groups. In addition, value of Forced Swim Test shows a significant decrease (p≤0.0001) in G2 group when compared with G1, G3 and G4 groups. On the other hand there is no significant (p≥0.0001) between G1, G3 and G4 groups. The