Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 205 Sonali et al. / Pharma Science Monitor 9(2), Apr-Jun 2018, 205-217 Pharma Science Monitor 9(2), Apr-Jun 2018 RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ATORVASTATIN, ASPIRIN, RAMIPRIL AND METAPROLOL SUCCINATE IN TABLET DOSAGE FORM Sonali M. Chaudhari * , Karan M. Prajapati, Shailesh V. Luhar, Sachin B. Narkhede Smt. B.N.B Swaminarayan Pharmacy College, Salvav, Vapi-396191, Gujarat. ABSTRACT A Simple and rapid reversed phase high performance liquid chromatography (RP-HPLC) method has been developed and Validated for the Simultaneous estimation of atorvastatin, aspirin, ramipril and metoprolol succinate in bulk and its dosage form. All the drugs were separated on a (250*4.6mm,) C18 column with a 5µm particle size. The mobile phase, which optimized through an experimental design, was a 90:10 (v/v) mixture of phosphate buffer (pH 4) an acetonitrile, pumped at a flow rate of 1ml/min. UV detection was performed at 210 nm. The retention time of atorvastatin, aspirin, ramipril and metoprolol succinate was found to be 8.013 min, 4.497 min, 7.240 min and 3.403 min respectively. The method was validated in the sample concentration ranges of 3-9µg/ml for atorvastatin, 22.5 9µg/ml-67.59µg/ml for aspirin, and 1.5- 4.59µg/ml for ramipril and 15-45µg/ml for metoprolol succinate. The LOD values were 0.3489µg/ml, 2.6739µg/ml, 0.2369µg/ml and 1.6279µg/ml while the LOQ values were 1.056µg/ml, 8.100µg/ml, 0.716 µg/ml and 4.931 µg/ml for atorvastatin, aspirin, and ramipril and metoprolol succinate. The recoveries for all four drugs were found to be above 98%. KEYWORDS: Liquid chromatography, HPLC, Simultaneous estimation, Atorvastatin, Aspirin, Ramipril and Metoprolol Succinate. INTRODUCTION Modern HPLC method development and discusses approaches for initial method development (column, detector, and mobile phase selection), method optimization to improve resolution, and emerging method development trends. Aspirin (ASP) is an analgesic and antipyretic, anti- inflammatory, anti-arthritic and anti-platelet drug. Chemically aspirin is 2-acetoxybenzoic acid. [1-4] Ramipril (RAM) is a potent and specific angiotensin converting enzyme (ACE) inhibitor. [1-4] Chemically ramipril is Cyclopenta[b]pyrrole-2 -carboxylic acid 1-[2-(ethoxycarboxyl-3- phenylpropyl) amino-1-oxopropyl] octahydro. [1-4] Metoprolol Succinate (MS) is a beta- adrenergic blocking agent, which reduces chest pain and lowers high blood pressure. Chemically Metoprolol Succinate is 1-[4-(2-methoxy ethyl) phenoxy]-3-[propan-2-ylamino] 2-propanol succinate. (1-4) Atorvastatin (ATR) is an anti-hyperlipoproteinemic agent that acts by inhibiting PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com