J PharmSciBioscientific Res. 2016 6(3):283-290 ISSN NO. 2271-3681 Patel K. S. et al 283 Development and Validation OF RP-HPLCMethod for the Simultaneous Estimation of Eplerenone and Torsemide in Pharmaceutical Dosage Form Krupali S. Patel 1 , Atul Bendale 2 , Shailesh V. Luhar 3 , Sachin B. Narkhede 4 1. M.PHARM Student, Smt. B.N.B Swaminarayan Pharmacy College, Salvav, Gujarat, India. 2. Assistant Professor of Department of Pharmaceutical Analysis, Smt. B.N.B Swaminarayan Pharmacy College, Salvav, Gujarat, India. 3. Head of department of Quality Assurance. Smt. B.N.B Swaminarayan Pharmacy College, Salvav, Gujarat, India. 4. Principal of Smt. B.N.B Swaminarayan Pharmacy College, Salvav, Gujarat, India. ABSTRACT: A simple, precise, accurate, rapid RP-HPLC Method and UV Spectrophotometric Methods were developed and validated for simultaneous estimation of Eplerenone and Torsemide in pharmaceutical dosage form.RP- HPLC was carried out by using Sheisedo C18 (250 * 4.6 mm, 5µm) column and Acetonitrile: Methanol: water (30:50:20 % v/v/v) as mobile phase, at 1.0 ml/min flow rate. Detection was carried out at 268 nm. Retention time was found to be 2.53 min and 3.27min for Torsemide and Eplerenone, respectively.RP-HPLC method was found to be linear over the range of 40-240µg/ml for Torsemide and 100-600µg/ml for Eplerenone. KEY WORDS: Eplerenone, Torsemide, RP-HPLC Method,Validation Article history: Received 01 April 2016 Revised 14 April 2016 Accepted 16 April 2016 Available online 20 April 2016 Citation: Patel K. S., Bendale A., Luhar S. V., Narkhede S. B. Development and Validation OF RP-HPLCMethod for the Simultaneous Estimation of Eplerenone and Torsemide in Pharmaceutical Dosage Form. J Pharm SciBioscientific Res. 2016, 6(3): 283-290 *For Correspondence: Krupali S. Patel M.PHARM Student, Smt. B.N.B Swaminarayan Pharmacy College, Salvav, Gujarat, India. (www.jpsbr.org) 1. INTRODUCTION [9 – 12] TORSEMIDE Torsemide is a loop diuretic drug, chemically it is 3-Pyridinesulfonamide, N- [[(1 methylethyl) amino] carbonyl]-4-[(3-methylphenyl) amino]-1-Isopropyl- 3-[(4-m-toluidino-3-pyridyl) sulfonyl] urea . It is useful in the treatment of hypertension or edema associated with congestive heart failure, renal disease and hepatic disease.Recently a formulation of Torsemide has been launched in market. In this formulation, Torsemide shows a synergistic effect with other combination. Torsemide was determined by several methods for the analysis including gaschromatography (GC), liquid chromatography with UV detection (LC–UV), HPTLC derivative spectrophotometric. Torsemide was determined with or without combination of several drugs by HPLC, spectrophotometry and HPTLC but literature survey revealed that no HPLC method has been reported yet for single estimation for Torsemide. The present study was aimed to develop a simple, rapid, precise, accurate, and selective chromatographic method for estimation of Torsemide oxalate in bulk and dosage forms with the use of buffer in the mobile phase in short duration. EPLERENONE Eplerenone has the chemical name Pregn-4-ene-7, 21-dicarboxylic acid, 9, 11-epoxy-17-hydroxy-3-oxo, γ-lactone, methyl ester (7α, 11α, 17α). It is off-