REV.CHIM.(Bucharest) ♦69♦No. 11 ♦2018 http://www.revistadechimie.ro 3037 Magnetic Stimulation on Human Blood Electromotive force analysis TEODORO CORDOVA FRAGA 1 *, DULCE MARIA MAGDALENO 1 , JOSE FRANCISCO GOMEZ AGUILAR 2 , BLANCA OLIVIAMURILLO 3 , MODESTO SOSA 1 , DUMITRU BALEANU 4,5 , RAFAEL GUZMAN CABRERA 6 1 Departamento de Ingenierra DCI, Universidad de Guanajuato campus Leon, Loma del Bosque N. 203, Loms del Campestre, 37150 Leon, GTO, Mexico 2 CONACyT- Centro Nacional de Investigacion y Desarrollo Tecnologico, Tecnologico Nacional de Mexico Interior Internado Palmira S/N, Palmira, 62490 Cuernavaca, MOR, Mexico 3 Unidad de Investigacion en Epidemiologia Clinica, UMAE N. 1 Bajio, IMSS, Blvd. Lopez Mateos Esq. Insurgentes, Los Paraísos, 37320 Leon, GTO, Mexico 4 Department of Mathematics, Cankaya University, Ankara, Turkey 5 Institute of Space Sciences, Magurele Bucharest, Romania 6 Division de Ingenierias, - DICIS, Universidad de Guanajuato campus Irapuato Salamanca, Salamanca, GTO, Mexico In this work a comparative theoretical analysis vs. experimental study on human blood under a magnetic field stimulation is presented. Twenty samples of leukoreduced human blood were stimulated with an alternant magnetic field using a Helmholtz coil system; this magnetic field induced an electromotive force in them. Theoretical calculations were performed for the induced electromotive force in a simple model of blood tissue under magnetic stimulation at frequencies: 50 Hz, 100 Hz, 800 Hz, and 1500 Hz. Experimental measurement was performed at the same frequencies for comparison purposes. Results show a high correlation between theoretical and experimental study, as well as effects of agglutination in the stimulated blood cells. Keywords: Alternating Magnetic field stimulation, Helmholtz coils, Induced electromotive force, Stimulated human blood Interactions between living systems and magnetic field (MF) on the environment cannot be avoided; therefore, it is important to widely study those interactions in order to understand any potential side effects. Earlier studies have shown the presence of proliferation, agglutination, growth and other effects when some culture cells are stimulated with applied magnetic field (AMF) at low or high frequencies. In particular, H.A. Perez et al. 2013 [1] showed the changes on cell motility in Entamoeba invades culture under stimulation with vortex of MF generated with a Rodin coil, they discussed that at low frequencies, the cells showed changes in their motility with rapid response compared to cells stimulated with frequencies greater than 400 Hz. Rodriguez De la Fuente, et al. (2008) [2] mentioned the effects of cell proliferation to the same group of cells when it is stimulated at 60 Hz. Nevertheless, they also suggest that the side effects of MF in cells are still considered different, for example, in 1993 Goodman [3] suggested that MF have effects on specific genes responsible for growth and even on certain calcium channels of the cell membrane that might affect cell growth. More recently H.A. Perez et al. 2013 [4], while he was working with human lymphocytes showed that, 20 days later, a considerable increase in the number of cells had increased the number of divisions of these. Blood cells have also been studied, in particular, Higashi et al (1997) [5] studied the orientation of erythrocytes under a static MF of 8.0 T where a parallel orientation to the field lines was observed, authors suggested that this is due to magnetic features of the cell membrane. Later, M. Sosa et al (2005) [6] studied the changes in the electrical properties of the blood when it was exposed to a magnetic field of 0.5 T finding a resistance increase of 10.4 % and a 1.9 % in capacitance; given the highly non-conductive membrane properties, they were able to explain these * email: theo@dci.ugto.mx changes based on ion currents induced in the interstitial medium and the charge distribution generated in the membrane surface because of ions that remain attached to it. Other authors have studied sedimentation and aggregation behavior when the erythrocytes are in a MF of the order of 6.0 T [7]. Physically, it is known that when a biological system is modeled as a conductor and it is stimulated by a MF, then by Faraday’s law, it is induced a electromotive force ( e m f) in the tissue which causes a flow of a current density through the biological system [8]. As part of this work, theoretical calculation for the induced emf in a simple model of blood tissue is presented. Also, the experimental evaluation in the blood samples magnetically stimulated at four frequencies with a Helmholtz coils system was also analyzed. Experimental part Materials and methods A Helmholtz coils system was used for the magnetic stimulation, it has two identical coils with 98 windings, a diameter of 20 ± 0.1 cm and a separation form one coil to the other of 10 ± 0.1 cm. Also it has a resistance of 233.28 Ω and an inductance of 5.06 mH. The coil system is controlled by LabVIEWO software. A signal, with frequency and time well defined, is controlled from the PC’s audio output directly to an audio amplifier model MIT-75AZ that increases the signal to 12 v RMS and then to the Helmholtz coil system. Additionally, a multimeter was connected to the circuit to record the current, I B , through the coil. In table 1 we show the values of electric current and magnetic field recorded with signals of different frequencies applied to the Helmholtz coil, such frequencies were selected from our previous experience that suggested proliferation changes in the blood cells.