Research Article
Rutin and Hesperidin Revoke the Hepatotoxicity Induced by
Paclitaxel in Male Wistar Rats via Their Antioxidant, Anti-
Inflammatory, and Antiapoptotic Activities
YasmineA.Ali ,
1
HananA.Soliman ,
1
MohamedAbdel-Gabbar ,
1
NohaA.Ahmed ,
2
Kandil A. A. Attia ,
3,4
Fatma M. Shalaby ,
5,6
El-Shaymaa El-Nahass ,
7
and Osama M. Ahmed
2
1
Biochemistry Department, Faculty of Science, Beni•Suef University, P.O. Box 62521, Beni•Suef, Egypt
2
Physiology Division, Zoology Department, Faculty of Science, Beni•Suef University, P.O. Box 62521, Beni•Suef, Egypt
3
Clinical Nutrition Department, College of Applied Medical Sciences, Jazan University, P.O. Box 114, Jazan 45142, Saudi Arabia
4
Department of Evaluation of Natural Resources, Environmental Studies and Research Institute, El•Sadat City University,
El•Sadat City 32897, Egypt
5
Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia
6
Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt
7
Department of Pathology, Faculty of Veterinary Medicine, Beni•Suef University, P.O. Box 62521, Beni•Suef, Egypt
Correspondene should be addressed to Osama M. Ahmed; osamamoha@yahoo.om
Received 20 July 2022; Revised 9 September 2022; Accepted 29 September 2022; Published 26 May 2023
Aademi Editor: Mohammed El•Magd
Copyright©2023YasmineA.Alietal.TisisanopenaessartiledistributedundertheCreativeCommonsAttributionLiense,
whih permits unrestrited use, distribution, and reprodution in any medium, provided the original work is properly ited.
Palitaxel, one of the most efetive hemotherapeuti drugs, is used to treat various aners but it is exeedingly toxi when used long•
term and an harm the liver. Tis study aimed to see if rutin, hesperidin, and their ombination ould protet male Wistar rats against
palitaxel(Taxol)•induedhepatotoxiity.AdultmaleWistarratsweresubdividedinto5groups(eahofsixrats). Tenormalgroupwas
orallygiventheequivalentvolumeofvehilesfor6weeks.Tepalitaxel•administeredontrolgroupreeivedintraperitonealinjetionof
palitaxel at a dose of 2mg/Kg body weight twie a week for 6 weeks. Treated palitaxel•administered groups were given palitaxel
similar to the palitaxel•administered ontrol group together with oral supplementation of rutin, hesperidin, and their ombination at
a dose of 10mg/Kg body weight every other day for 6 weeks. Te treatment of palitaxel•administered rats with rutin and hesperidin
signifantly redued palitaxel•indued inreases in serum alanine transaminase, aspartate transaminase, latate dehydrogenase, al•
kaline phosphatase, and gamma•glutamyl transferase ativities as well as total bilirubin level and liver lipid peroxidation. However, the
levels of serum albumin, liver glutathione ontent, and the ativities of liver superoxide dismutase and glutathione peroxidase inreased.
Furthermore, palitaxel•indued harmful hepati histologial hanges (entral vein and portal area blood vessel ongestion, fatty
hanges, and moderate neroti hanges with foal nulear pyknosis, foal mononulear infltration, and Kupferellproliferation) were
remarkably enhaned by rutin and hesperidin treatments. Moreover, the elevated hepati proapoptoti mediator (aspase•3) and pro•
infammatoryytokine(tumornerosis fator•α)expressionsweredereasedbythethreetreatmentsinpalitaxel•administeredrats.Te
otreatment with rutin and hesperidin was the most efetive in restoring the majority of liver funtion and histologial integrity.
Terefore, rutin, hesperidin, and their ombination may exert hepati protetive efets in palitaxel•administered rats by improving
antioxidant defenses and inhibiting infammation and apoptosis.
1.Introduction
Palitaxel,whihstabilizesmirotubulesandinhibitstheir
depolymerization during ell division, is one of the most
widely used hemotherapy drugs [1–4]. Te ative om•
pound seletion program founded by the National Caner
Institutein1981provedthatpalitaxelwastheonlyative
biologial ingredient that falls within this ategory and
Hindawi
Evidence-Based Complementary and Alternative Medicine
Volume 2023, Article ID 2738351, 17 pages
https://doi.org/10.1155/2023/2738351