Vol. 14 | No. 2 |672-675| April - June | 2021 ISSN: 0974-1496 | e-ISSN: 0976-0083 | CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in Rasayan J. Chem., 14(2), 672-675(2021) http://dx.doi.org/10.31788/ RJC.2021.1425826 This work is licensed under a CC BY 4.0 license. ANTI-Escherichia coli NON-PATHOGENIC STRAIN ACTIVITY OF COMPLEX COMPOUND BASED ON COPPER(II) AND 2,4,5- TRIPHENYL-1H-IMIDAZOLE T. H. Sucipto 1 , W. Setyarini 2 , F. Martak 3 and D. Raharjo 2,4, 1 Institute of Tropical Disease, Universitas Airlangga, Campus C UNAIR, Mulyorejo Surabaya, East Java 60115, Indonesia 2 Gastroenteritis Study Group, Institute of Tropical Disease, Universitas Airlangga, Campus C UNAIR, Mulyorejo Surabaya, East Java 60115, Indonesia 3 Department of Chemistry, Institut Teknologi Sepuluh Nopember, Raya ITS Surabaya, East Java 60111, Indonesia 4 Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga Campus C UNAIR, Jl. Mulyorejo Surabaya, East Java 60115, Indonesia  Corresponding Author: dadik_tdc@yahoo.co.id ABSTRACT The complex of Copper(II) binding 2,4,5-triphenyl-1H-imidazole ligand is a new copper complex and exhibits therapeutic properties to bacteria. The present observation is focused on anti-Escherichia coli non-pathogenic strain using disc diffusion method. Copper(II)-2,4,5-triphenyl-1H-imidazole complex compound showed anti-Escherichia coli non-pathogenic strain activity with a range of inhibition zone 14.51-17.37 mm and compared with free metal Cu(II) less reactive with a range of inhibition zone 10.57-27.16 mm, but more no toxic in the Vero cells with CC50 44.74 µg/ml. Keywords: Copper(II), 2,4,5-triphenyl-1H-imidazole, Complex Compound, Antibacterial RASĀYAN J. Chem., Vol. 14, No.1, 2021 INTRODUCTION 2,4,5-triphenyl-1H-imidazole plays vital parts for supramolecular congregations since it can give a bidentate N-donor site for chelating with ions of the metal to create bridge ligands, imidazole is a five- membered ring aromatic compound. It is composed of 3 carbon atoms and 2 nitrogen atoms. 1,2,3 Imidazole derivatives are known to possess desirable antibacterial, 4-8 antifungal, 6 anticancer, 9-11 and antiviral properties, 12,13 also 2,4,5-triphenyl-1H-imidazole are exceptionally imperative ligands in organometallic chemistry. Moreover, a few of their complexes bind to DNA. Imidazole antibiotic is pharmaceuticals that are commonly used in human medications and veterinary drugs. 14 Imidazole 2-aldoximes derivatives were reported anti-Escherichia coli with inhibition zone from 12.4 ± 0.71 to 21.8 ± 0.9 mm). 15 The importance of metal ions in the vital function of living organisms, from mammalians to bacteria, has been widely recognized. They are incorporated into enzymes and cofactors that are required for various life processes. Copper is a basic following component for numerous organic forms, found in enzyme assortment, counting the copper centers of cytochrome C oxidase, the Cu-Zn-containing superoxide dismutase enzyme, and it is the central metal within the oxygen-carrying hemocyanin pigment. 16 Growth inhibitory effects on common urinary pathogens have been reported, but uropathogenic Escherichia coli (UPEC) are less sensitive to nitric oxide than non-pathogenic strains of Escherichia coli. 17 In general, the synthesized metal complexes have higher activities of biological to free ligands. Metal complexes excessively aggravate the respiratory process of cells and enhance the fusion of proteins, limiting aiding the development of life forms. In this study, the azomethine linkage (-C=N-) within the synthesized complexes displays broad natural action due to expanding the molecules liposolubility in microorganism crossing cell membrane. 18