Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Wed, 12 Dec 2018 18:32:20 Ogataea mangiferae sp. nov., a methylotrophic yeast isolated from mango leaves Ana Raquel O. Santos, 1 Elisa S. Faria, 1 Marc-Andre ´ Lachance 2 and Carlos A. Rosa 1 Correspondence Carlos A. Rosa carlrosa@icb.ufmg.br 1 Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil 2 Department of Biology, University of Western Ontario, London, Ontario, N6A 5B7, Canada Five strains of a novel methanol-assimilating yeast species were isolated from mango (Mangifera indica) leaves collected at the campus of the Federal University of Minas Gerais in Brazil. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit of the rRNA gene showed that this species belongs to the Ogataea clade and is related to O. allantospora, O. chonburiensis, O. dorogensis, O. kodamae, O. paradorogensis and Candida xyloterini (Ogataea clade). The novel species differs in the D1/D2 domains of the large subunit of the rRNA gene by 12 to 40 substitutions from these Ogataea species. The name Ogataea mangiferae sp. nov. is proposed for this novel species. The type strain of Ogataea mangiferae sp. nov. is UFMG-CM-Y253 T ( 5 CBS 13492 T ). The Mycobank number is MB 811646. Yeast species of the Ogataea clade share the ability to grow with methanol as the sole carbon source (Kurtzman, 2011; C ˇ adez ˇ et al., 2013). In this clade, only O. salicorniae does not assimilate methanol (Kurtzman, 2011). In addition to the 31 known species of Ogataea listed by Kurtzman (2011), nine species belonging to this clade were described recently (C ˇ adez ˇ et al., 2013). Most species of Ogataea are associated with rotting wood, the external surfaces of leaves or insect frass, or as minor components of yeast communities on bodies of water and olive oil (Kurtzman 2011; Koowadjanakul et al., 2011; Limtong et al., 2013; C ˇ adez ˇ et al., 2013). External surfaces of leaves are a known substrate for yeast growth (Fonseca & Ina ´cio, 2006). Nutrients leaching from plant leaves include sugars, organic acids, amino acids, methanol and salts (Fonseca & Ina ´cio, 2006; Pe ´ter et al., 2007). Several yeast species exploit these substrates, and methanol-assimilating species form minor com- ponents of these communities (Pe ´ter et al., 2007). Species of Ogataea were previously isolated from substrates, and it is suggested that these species utilize the methanol pro- duced by pectin demethylation inside leaves and emitted through stomata (Fall & Benson, 1996; Pe ´ter et al., 2007). During a study of the yeast communities associated with mango (Mangifera indica) leaves in the campus of the Fed- eral University of Minas Gerais in Brazil, five strains of a novel methanol-assimilating yeast species were found. Sequence analysis of the D1/D2 domains and ITS region of the rRNA gene showed that these strains represent a novel yeast species closely related to O. allantospora, O. chonburiensis, O. dorogensis, O. kodamae, O. paradorogen- sis and Candida xyloterini (Ogataea clade). In this work, we describe the species as Ogataea mangiferae sp. nov. Strains UFMG-CM-Y253 T (5CBS 13492 T ), 320, 321, 322 and 410 were isolated from external surfaces of Mangifera indica leaves collected at the campus of the Federal University of Minas Gerais at the city of Belo Horizonte, Minas Gerais state, Brazil. Fifty-six trees were sampled for this study. The yeast strains were isolated in yeast nitrogen base (YNB) supplemented with 0.5 % (v/v) methanol according to a two-step enrichment procedure described by Dlauchy et al. (2003) and Pe ´ter et al. (2007). Mature leaves without sign of disease were collected from August to November of 2008. Approximately 20 g of leaves from individual trees were collected and processed using the two-step enrichment procedure. The leaves of each individ- ual tree were put in 250 ml flasks containing 50 ml of the enrichment broth and incubated on a rotary shaker at room temperature (25¡3 8C) for 5–7 days. After the growth, 1 ml was inoculated in a flask containing 10 ml of the enrichment medium. After this second growth period, one loopful of the enriched culture was streaked on 0.5 % (v/v) methanol-YNB agar and incubated at 25 8C until yeast colonies developed. The different yeast morphotypes were purified by repeated streaking on yeast mould (YM) Abbreviation: ITS, internal transcribed spacer. The GenBank/EMBL/DDBJ accession number for the sequence of the ITS region and D1/D2 domain of the large subunit of the rRNA gene of strain UFMG-CM-Y253 T is KF585022. International Journal of Systematic and Evolutionary Microbiology (2015), 65, 1855–1859 DOI 10.1099/ijs.0.000194 000194 G 2015 IUMS Printed in Great Britain 1855