International Journal of Clinical Biochemistry and Research 2022;9(4):291–294 Content available at: https://www.ipinnovative.com/open-access-journals International Journal of Clinical Biochemistry and Research Journal homepage: https://www.ijcbr.in/ Original Research Article Diagnostic role of different biochemical parameters in pleural effusion Jaspreet Singh 1, *, Gitanjali 1 , Khushdeep Singh 1 , Rincal Saini 1 , Umesh Kumar 1 , Shiv Sharma 1 , Harvinder Singh 1 1 Dept. of Biochemistry, Baba Farid University of Health Sciences, Punjab, India ARTICLE INFO Article history: Received 20-10-2022 Accepted 08-11-2022 Available online 31-12-2022 Keywords: Pleural effusion LDH PH Glucose Triglycerides Cholestrol Creatinine Amylase ADA ABSTRACT Introduction: A pleural effusion, an excessive accumulation of fluid in the pleural space, indicates an imbalance between pleural fluid formation and removal. For diagnosing and treatment plan, pleural effusions have to be classified into transudate and exudate. Aim: The aim of present study was to analyze various biochemical parameters (LDH, pH, Glucose, Triglycerides, Cholesterol, Creatinine, Amylase and ADA) in pleural fluid and to correlate these Biochemical parameters with diagnosis of the patients. Materials and Methods: The study was a hospital based descriptive study. The study was conducted over a period of one year on 100 samples. Total 100 samples were enrolled in the study. Both serum and pleural fluid samples were collected and quantitatively analyzed using semi-automated analyzer. Results: The results of the present study shows that The Mean±SD of concentration of glucose in pleural fluid was 65.69±14.13 mg/dl, total protein 3.53±1.53g/dl, albumin 1.64±0.85g/dl, cholesterol 58.9±13.05mg/dl, triglycerides 68.7±10.47mg/dl, creatinine 1.73±0.96mg/dl, amylase 49.56±17.78IU/L, LDH 299.82±65.46 U/L and ADA was 49.56±17.78U/L. Conclusion: It was concluded that Biochemical parameters play important role in diagnosing Pleural effusions. These markers when used collectively their diagnostic efficacy is greatly increased. The SEAG is superior to Light’s criteria in identifying the transudative effusions. It is also observed that Light’s criteria identified exudative effusions better than SEAG. This is an Open Access (OA) journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. For reprints contact: reprint@ipinnovative.com 1. Introduction All healthy humans have a small amount of pleural fluid that lubricates the space and facilitates normal lung movements during respiration. The pleural fluid normally provides lubrication between the parietal and visceral membranes and the organs contained within the space. 1 A pleural effusion, an excessive accumulation of fluid in the pleural space, indicates an imbalance between pleural fluid formation and removal. Accumulation of pleural fluid is not a specific disease, but rather a reflection of * Corresponding author. E-mail address: chauhanjaspreetsingh@gmail.com (J. Singh). underlying pathology. Pleural effusions accompany a wide variety of disorders of the lung, pleura, and systemic disorders. Therefore, a patient with pleural effusion may present not only to a pulmonologist but to a general internist, rheumatologist, gastroenterologist, nephrologist, or surgeon. To treat pleural effusion appropriately, it is important to determine its cause. 2 For diagnosing and treatment plan, pleural effusions have to be classified into transudate and exudate. The routine pleural fluid evaluation usually includes determination of protein, pH, lactate dehydrogenase, glucose, and albumin levels, with adenosine deaminase levels and cell count for differential and cytological examination. 3 If the diagnosis is not https://doi.org/10.18231/j.ijcbr.2022.057 2394-6369/© 2022 Innovative Publication, All rights reserved. 291