Advances in Animal and Veterinary Sciences September 2022 | Volume 10 | Issue 9 | Page 1933 INTRODUCTION H yperlipidemia, T2D, and cardiovascular disease are all conditions that afect the body’s lipid levels and related to obesity and insulin resistance (Fried et al., 2008; Yalniz et al., 2007; Pagotto et al., 2008; Lavie et al., 2008). SREBP-1c gene transcription in mice and humans was regulated by the efects of insulin and the presence of cel- Research Article Abstract | Te correlation between obesity and metabolic disorders like Type 2 diabetes, cardiovascular disease, and several types of cancer has made obesity a challenge for world health. Te current study’s objective was to assess the antioxidant capacity of cranberry extract in high-fat diet-induced obesity. For eight weeks, 36 white albino rats weighing 197±10 g were selected at random to fve groups: Group I was administered a regular diet; Group II was fed a high-fat diet; Group III was given a high-fat diet plus cranberry extract (75 mg/kg); Group IV was fed a high- fat diet with cranberry extract (150 mg/kg); and Group V was fed a high-fat diet plus metformin (500 mg/kg). Te lipid profle was determined using blood samples, and liver tissue samples were used to determine glucose, insulin, leptin, TC, TG, HDL-C, ALT, AST, MDA, TNF-α, PCO. Te HFD administrated rats resulted in increased total body weight, glucose, insulin, leptin, cholesterol, triglycerides, MDA, ALT, AST, TNF- α and PCO levels, as well as SREBP1 gene expression, according to the fndings. However, our fndings revealed a signifcant reduction in HDL-C, GSH, CAT and PON 1 enzyme levels, the expression of the PPAR– α, CPT I and ACO gene in HFD treated rats. Also, administration of cranberry extract and metformin signifcantly normalized body weight, blood glucose, insulin, leptin, total cholesterol, triglycerides, HDL-C, TBARS, GSH, CAT, ALT, AST, TNF- and PCO and PON 1 as well as SREBP1, PPAR– α, CPT I and ACO levels. Te biochemical and PCR results are supported by electrophoretic patterns and histological evidence. Conclusions: Te fndings imply that cranberry extract could be used as a new pharmaceutical drug in the treatment of obesity. Keywords | Cranberry extract, Insulin, Leptin, Total cholesterol, Paraoxonase1 AYSAM FAYED 1 , MOHGA A IBRAHEM 2 , SOHA A. HASSAN 3 , MOHAMMED ABDALLA HUSSEIN 4* , TAMER ROSHDY 1 Cranberry Extract; as a Promising Functional Food to Regulate SREBP1/PPAR–α/CPT-1/ACO Signaling Pathways in HFD- Induced Obesity in Rats Received | June 07, 2022; Accepted | July 17, 2022; Published | August 16, 2022 *Correspondence | Mohammed Abdalla Hussein, Biochemistry Department, Faculty of Applied Medical Sciences, October 6 University, Sixth of October City, Egypt; Email: prof.husseinma@o6u.edu.eg Citation | Fayed A, Ibrahem MA, Hassan SA, Hussein MA, Roshdy T (2022).Cranberry extract; as a promising functional food to regulate srebp1/ppar–α/cpt-1/ aco signaling pathways in hfd-induced obesity in rats. Adv. Anim. Vet. Sci. 10(9): 1933-1944. DOI | http://dx.doi.org/10.17582/journal.aavs/2022/10.9.1933.1944 ISSN (Online) | 2307-8316 Copyright: 2022 by the authors. Licensee ResearchersLinks Ltd, England, UK. Tis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY ) license (https://creativecommons. org/licenses/by/4.0/). 1 Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Menoufa, Egypt; 2 Med. Labs. Department, Faculty of Applied Medical Sciences, October 6 University, Sixth of October City, Egypt; 3 Basic Science Department, Faculty of Dentistry, October 6 University, Sixth of October City, Egypt; 4 Biochemistry Department, Faculty of Applied Medical Sciences, October 6 University, Sixth of October City, Egypt.