Letter to the Editor
Nephron 1999;81:366–367
Effect of Seasonal Changes on the
Cyclosporine A Blood Levels in Renal Transplant
Recipients during Childhood
Salih Kavukçu
a
Alper Soylu
a
Mehmet Türkmen
a
Seymen Bora
b
Hülya Güven
c
Hüseyin Gülay
b
Departments of
a
Pediatrics,
b
General Surgery and
c
Pharmacology, Dokuz Eylül University Medical Faculty, I
˙
zmir,
Turkey
Dr. Salih Kavukçu
Department of Pediatrics
Dokuz Eylül University Medical Faculty
TR–35280 I
˙
zmir (Turkey)
Tel./Fax +90 232 231 2610
ABC
Fax + 41 61 306 12 34
E-Mail karger@karger.ch
www.karger.com
© 1999 S. Karger AG, Basel
0028–2766/99/0813–0366$17.50/0
Accessible online at:
http://BioMedNet.com/karger
Dear Sir,
The food consumption of children is re-
lated to seasonal changes in developing
countries. Some natural substances like fla-
vonoids, found in various fruits and vegeta-
bles, have been reported to inhibit the cyto-
chrome P-450 oxidase system which is re-
sponsible for the major part of the metabo-
lism of cyclosporine A (CsA) [1, 2]. The
blood CsA level has been reported to be high-
er when the drug was administered with
juices of flavonoid-containing fruits, espe-
cially grapefruit [3]. Our region, located on
the east shore of the Aegean Sea, has the
characteristic Mediterranean climate and
many fruits and vegetables, like grapefruit,
leek and lettuce, rich in flavonoids, are culti-
vatable in this region, especially in the win-
ter months.
We wanted to evaluate retrospectively
the relationship of seasonal changes to CsA
blood levels in children with renal trans-
plants living in a Mediterranean climate.
Starting 3 months after renal transplant, 7
children (6 males, mean age 13.5 B 1.1
years; mean posttransplantation follow-up
35.3 B 3.9 months) were evaluated retro-
spectively to study their monthly blood CsA
levels. All of the patients were treated with
similar doses of CsA (5 mg/kg/day), azathio-
prine (1 mg/kg/day) and prednisolone (10
mg/day) after this period. In addition, pa-
rameters related to the fluid and electrolyte
balance, like serum creatinine and electro-
lytes, were evaluated to exclude the effect of
alterations in these parameters on the blood
level of the drug. Then, winter (December to
February) and summer (June to August) val-
ues were compared.
Serum creatinine and electrolytes were
normal in all of the patients and did not dif-
fer seasonally. Blood CsA levels were higher
during the summer in 6 of 7 patients (85.7%)
and the cumulative CsA values were signifi-
cantly higher during the summer in compari-
son to the winter (123.7 B 6.8 and 108.6 B
8.2 ng/ml, respectively, p ! 0.02). During the
winter the mean blood CsA levels were less
than the therapeutic range (100–150 ng/ml)
in 2 of 7 patients (28.5%), while all of the
patients had levels within therapeutic range
during the summer, except for 1 patient who
had even higher values (table 1).
CsA may cause arteriolar changes, inter-
stitial fibrosis and progressive renal failure
[4] and thus, its blood level should be kept
within the therapeutic range (100–150 ng/ml
3 months after transplantation). CsA is me-
tabolized by the cytochrome P-450 mixed
function oxidase system which is effected by
various drugs and substances like steroids
Table 1. Mean whole-blood cyclosporine A (CsA, normal therapeut-
ic range 100–150 ng/ml) levels determined during the winter and
summer months and comparison of these values in each patient
(mean B SE)
Patient
No.
CsA level, ng/ml
summer winter
p
1 118.1B7.5 96.1B15.7 1 0.05
2 105.0B17.5 121.0B7.7 1 0.05
3 118.2B21.4 114.1B20.3 1 0.05
4 102.8B18.7 64.6B9.6 1 0.05
5 146.5B18.2 125.2B28.7 1 0.05
6 171.0B22.7 130.0B21.3 1 0.05
7 148.6B24.5 133.1B29.3 1 0.05
Cumulative 123.7B6.8 108.6B8.2 ! 0.05
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