Advances in Pharmacology and Pharmacy 11(1): 57-65, 2023 http://www.hrpub.org DOI: 10.13189/app.2023.110106 In vitro Anti-inflammatory, Anti-diabetic and Anticancer Properties of Copper Nanoparticles Synthesized by Medicinal Plant Leucas aspera (Willd) Running Title: In vitro Pharmocological Activities of Leucas aspera Copper Nanoparticles K. Bhuvaneswari, KM Jerun Nisha, K. Kalimuthu * , M. Lakxmi Kiruthika, E. Shanthi Priya, Sathiya Sheela D. PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore – 641 018, Tamil Nadu, India Received July 12, 2022; Revised November 3, 2022; Accepted November 15, 2022 Cite This Paper in the Following Citation Styles (a): [1] K. Bhuvaneswari, KM Jerun Nisha, K. Kalimuthu, M. Lakxmi Kiruthika, E. Shanthi Priya, Sathiya Sheela D. , "In vitro Anti-inflammatory, Anti-diabetic and Anticancer Properties of Copper Nanoparticles Synthesized by Medicinal Plant Leucas aspera (Willd)," Advances in Pharmacology and Pharmacy, Vol. 11, No. 1, pp. 57 - 65, 2023. DOI: 10.13189/app.2023.110106. (b): K. Bhuvaneswari, KM Jerun Nisha, K. Kalimuthu, M. Lakxmi Kiruthika, E. Shanthi Priya, Sathiya Sheela D. (2023). In vitro Anti-inflammatory, Anti-diabetic and Anticancer Properties of Copper Nanoparticles Synthesized by Medicinal Plant Leucas aspera (Willd). Advances in Pharmacology and Pharmacy, 11(1), 57 - 65. DOI: 10.13189/app.2023.110106. Copyright©2023 by authors, all rights reserved. Authors agree that this article remains permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Abstract Introduction: The present study focuses on the use of Leucas aspera aqueous extract as a reduction agent for the synthesis of copper nanoparticles (CuNPs). The entire plant is employed in conventional medicine and as an insecticide. Scientific research has been done on the plant’s potential as an anti-inflammatory, analgesic, and wound-healing agent. Intoxication by cobra venom also makes use of it. This study was intended to explore the in vitro anti-inflammatory, antidiabetic and anticancer activities of copper nanoparticles. Methods: Protein denaturation and membrane stabilization of human red blood cells (HRBC) were used to assess the in vitro anti-inflammatory activity. The antidiabetic activity of the CuNPs was evaluated by inhibiting both amylase and glucosidase in vitro, whereas anticancer activity against vero and MCF-7 cell line. Results: The Leucas aspera copper nanoparticles (LACuNPs) showed a dose dependent -inflammatory activity. The highest inhibition percentage in protein denaturation is 70.30±1.72% and 67.68±1.41% in HRBC membrane stabilization at 1000μg/ml concentration. In vitro antidiabetic activity exhibited 76.03 ± 1.14 of α-amylase and 71.54 ± 1.55 at α-glucosidase inhibition assay. The in vitro anti-cancer study revealed 50% apoptosis (IC 50 ) of LACuNPs treated vero and MCF7 are displayed as 248.84±0.01 μg/ml and 137.39±1.51, respectively. Conclusion: The results clearly demonstrated the application of biosynthesized LACuNPs as potential anti-inflammatory, anti-diabetic, and anticancer agents in addition to their environmental friendliness. Keywords In vitro Anti-inflammatory, Copper Nanoparticles, HRBC, MCF-7, α-glucosidase 1. Introduction Nanotechnology has recently attracted a lot of attention due to its adaptable qualities and possible uses in contemporary material science. Owing to the special characteristics of nanomaterials compared to bulk