*Correspondence: ari.asnani@unsoed.ac.id (Received: November 18, 2021; accepted: January 4, 2022) Citaton: Asnani A, Purwant A, Bakrudin WA, Anjarwat DU. The Producton of Streptomyces W-5B Extract for Antbioflm against Methicillin-resistant Staphylococcus aureus. J Pure Appl Microbiol. 2022;16(1):337-346. doi: 10.22207/JPAM.16.1.23 © The Author(s) 2022. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Asnani et al. | J Pure Appl Microbiol | 16(1):337-346 | March 2022 Artcle 7441 | htps://doi.org/10.22207/JPAM.16.1.23 Published Online February 5, 2022 Print ISSN: 0973-7510; E-ISSN: 2581-690X ReseARCh ARtiCle OPeN ACCess www.microbiologyjournal.org 337 Journal of Pure and Applied Microbiology The Producton of Streptomyces W-5B Extract for Antbioflm against Methicillin-resistant Staphylococcus aureus Ari Asnani 1 *, Azizah Purwant 1 , Windy Amalia Bakrudin 1  and Dwi Utami Anjarwat 2  1 Department of Chemistry, Faculty of Mathematcs and Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia. 2 Department of Microbiology, Faculty of Medicine, Universitas Jenderal Soedirman, Purwokerto, Indonesia. Abstract The ability of Methicillin-resistant Staphylococcus aureus (MRSA) to form bioflms is one of the triggering factors for the emergence of MRSA resistance to antbiotcs. Streptomyces W-5B has shown potency as an antbacterial producer against MRSA. However, the producton of microbial bioactve compounds is strongly afected by the source of nutrients in the fermentaton medium. Therefore, the objectve of this study was to determine the optmal sources of carbon and nitrogen for the producton of bioactve compounds with antbioflm actvites. The research method included cultvatng Streptomyces W-5B, extract producton, and variaton of carbon (glucose, sucrose, starch) and nitrogen (casein, peptone, urea) sources for fermentaton medium. Antbioflm actvites were measured based on inhibiton of bioflm formaton and bioflm degradaton tests using the microtter plate method with a crystal violet stain. The results showed that the highest inhibiton of bioflm formaton was 68.206 ± 1.750% afer 12 days of incubaton in a fermentaton medium containing sucrose and urea. Meanwhile, the highest bioflm degradaton was 73.023 ± 1.972% afer nine days of incubaton on a fermentaton medium containing starch and urea. These fndings indicated that Streptomyces W-5B has the potency to produce antbioflm extract against MRSA. Keywords: Antbioflm, carbon source, MRSA, nitrogen source, Streptomyces