MI-SPE, M-SPE AND M-SPD RECENT APPLICATION ON SOLID PHASE EXTRACTION FOR COMPOUND EXTRACTION OF COMPLEX MATRICES Review Article SHARIMINA VENU GOPALAN, ALIYA NUR HASANAH Pharmaceutical Analysis and Medicinal Chemistry Department, Faculty of Pharmacy, Universitas Padjadjaran Email: Received: 06 Feb 2019, Revised and Accepted: 28 Mar 2018 aliya.n.hasanah@unpad.ac.id ABSTRACT Solid Phase Extraction is a method used for extraction, purification of compounds contained in complex matrices and purification of samples in the fields of industry, pharmacy, and toxicology analysis. This review represents the recent advances of solid phase extraction from different mode of separation which are the most commonly used in recent days such as molecular imprinting solid phase extraction (MISPE), magnetic solid phase extraction (MSPE) and matrix solid phase dispersion (MSPD). In this review, the applications of MISPE in the analysis of agrochemicals such as herbicides, fungicides and insecticides are summarized. This review also states preparation of various magnetic composites (MSPE) based on graphene and its application as adsorbents in pre concentrating natural mixes, organic macromolecules. Besides this, this review summarizes the application of MSPD in biological, food, and environmental samples, including both organic and inorganic analyte. Keywords: Solid phase extraction, Recent advances, Molecular imprinting solid phase extraction, Magnetic solid phase extraction, Matrix solid phase dispersion © 2019 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) DOI: http://dx.doi.org/10.22159/ijap.2019v11i3.32410 A method utilized for extraction, sanitization of compounds contained in complex matrices and purification of samples in the fields of industry, pharmacy, and toxicology analysis is called strong stage extraction. It is the most generally utilized analytical extraction strategies and can facilitate the analysis of a compound from biological materials such as blood, urine, water, etc. which contain many matrices. The problem that often arises is when there is a sample whose composition is unknown but is very complex and contains so many chemical components in the form of liquid and the presence of solid particles that expand inside. Therefore, we need a technique that can analyze the specific compounds contained in a sample [1]. INTRODUCTION Solid Phase Extraction (SPE) is a relatively new methodology but SPE is fast developing as the main tool for sample pre-treatment or for clean-up of dirty samples, for example, samples that have high matrix content such as salts, proteins, polymers, resins, etc. Filtration, homogenization, precipitation, chemical reactions, solvent exchange, concentration, matrix removal, solubilization are components that can be used singly or in combination to obtain samples with forms that are compatible with the analysis tools needed [1]. SPE has the advantage when compared to liquid-liquid extraction that is by using SPE the extraction process becomes more perfect, the separation of analyte from the matrix becomes more efficient, reducing the organic solvents used. SPE is an efficient separation process so that high recovery (>99%) is easier to achieve when compared to liquid-liquid extraction. In liquid-liquid extraction, several extractions are still needed to obtain high recovery, whereas with SPE only one-step is needed. Moreover, SPE has advantages and has been widely used because it can use various stationary phases, fast and simple procedures and high throughput. The main advantage of SPE is its easy use, fast time and generally only requires a small volume of extraction solvents [2]. To improve selectivity in sample analysis, the current SPE method are combined with other methods such as chromatography (GC-MS) UV-Vis Spectrophotometer, HPLC, Gas and Mass Chromatography. According to Barnes et al., the combination of chromatography and SPE can be used more simply and effectively in purification, sample analysis. The advantages when compared to other methods such as HPLC, NMR, and MS are low costs, the ability and sample analysis are quite good Methodology [2]. Our review is based on the literature obtained from the PubMed and Scopus databases using the keywords “molecular imprinted solid phase extraction”, “matrix solid phase dispersion” and “magnetic solid phase extraction”. The specific keywords related to application of molecular imprinted solid phase extraction, matrix solid phase dispersion and magnetic solid phase extraction used were “molecular imprinted solid phase extraction in complex matrices,” “magnetic solid phase dispersion in food,” “molecular imprinted solid phase extraction in biological fluid,” and “matrix solid phase dispersion in pharmaceuticals. The following exclusion criteria were applied: reviews, opinions or commentaries, non-English language, and unrelated topics such as other type of solid phase extraction beside molecular imprinted solid phase extraction, magnetic solid phase extraction and matrix solid phase dispersion. Different mode of separatation The following are the frequently utilized method of detachment in solid phase extractions in current days dependent on the articles, for example, molecular imprinted solid phase extraction (MI-SPE), Magnetic SPE (MSPE) and matrix solid phase dispersion. In the new advancements of test readiness procedures, molecularly imprinted polymers (MIPs) have picked up enthusiasm as a novel sort of sorbent with alluring properties. Such polymers may move toward becoming supplement to antibodies to be utilized in pesticide assurance [3, 4]. Magnetic solid phase extraction has been utilized as pretreatment procedure for the examination of a few mixes due to its points of interest when it is contrasted and great strategies. Magnetic solid phase extraction limits the utilization of additional steps, for example, precipitation, centrifugation, and filtration that diminishes the control of the example [5-77]. Uses of MSPD have secured natural, nourishment, and ecological examples, including both organic and inorganic analyte. Matrix solid phase dispersion (MSPD) has turned out to be a proficient example arrangement method for solid, semi-solid, and sticky examples [8]. Molecular imprinting solid phase extraction The molecular printing technique enables synthetic polymers to be prepared for a template molecule with specific binding sites. This can be achieved if the target acts as a molecular template during the polymerization process. Monomers with functional groups are International Journal of Applied Pharmaceutics ISSN- 0975-7058 Vol 11, Issue 3, 2019