original article
Dental Press Endod. 2015 May-Aug;5(2):26-32 © 2015 Dental Press Endodontics 26
Evaluation of rat tissue response to Guedes-Pinto and
calcium hydroxide pastes by morphological analysis of
repair in rat’s tooth socket
ABSTRACT
In order to evaluate late biological response of two types
of filling material of deciduous teeth, calcium hydroxide
and Guedes-Pinto pastes, the implantation technique in
rat sockets was employed. For morphological analysis,
carried out after 7 and 28 days, 48 rats had their maxil-
lary right central incisor removed, and a polyethylene
tube implant containing one of the pastes was placed
into the socket. On the 7
th
day, calcium hydroxide paste
caused greater edema than Guedes-Pinto paste, and
both types of material showed connective tissue with
fibroblasts and blood vessels, with macrophages and
lymphocytes. On the 28
th
day, calcium hydroxide paste
had connective tissue with a large amount of macro-
phages and lymphocytes near the material, whereas
in further areas, it had neofomed trabeculae. For the
Guedes-Pinto paste, there were neoformed bone tra-
beculae nearby. The latter were found in larger amounts
in areas further from the material surface. Guedes-Pinto
paste caused less edema and provided less interference
in the repair process.
Keywords: Deciduous tooth. Calcium hydroxide. Root
canal therapy. Root canal filling material.
1
PhD professor of Pediatric Dentistry, Universidade do Oeste Paulista (UNOESTE), School of
Dentistry, Presidente Prudente, São Paulo, Brazil.
2
MSc in Animal Sciences, Universidade Estadual do Mato Grosso do Sul (UFMS), Aquidauana,
Mato Grosso do Sul, Brazil.
3
Professor of Pediatric Dentistry, Universidade do Oeste Paulista (UNOESTE), School of
Dentistry, Araçatuba, São Paulo, Brazil.
4
PhD professor of Endodontics, Universidade Estadual Paulista Júlio de Mesquita Filho
(UNESP), School of Dentistry, Araçatuba, São Paulo, Brazil.
Contact address: Karine Takahashi
Rua Francisco Scardazzi, 350, São Judas – CEP: 19.023-190
Presidente Prudente/SP, Brazil – E-mail: karine@unoeste.br
Submitted: July 25, 2015. Revised and accepted: August 05, 2015.
How to cite this article: Takahashi K, Maltempi Filho P, Cunha RF, Dezan Junior E.
Evaluation of rat tissue response to Guedes-Pinto and calcium hydroxide pastes by
morphological analysis of repair in rat’s tooth socket. Dental Press Endod. 2015 May-
Aug;5(2):26-32.
DOI: http://dx.doi.org/10.14436/2358-2545.5.2.026-032.oar
» The authors report no commercial, proprietary or inancial interest in the prod-
ucts or companies described in this article.
Karine TAKAHASHI
1
Paulo MALTEMPI FILHO
2
Robson Frederico CUNHA
3
Eloi DEZAN JUNIOR
4
DOI: http://dx.doi.org/10.14436/2358-2545.5.2.026-032.oar