Please cite this article in press as: Cruz ÉP, et al. Clinical, biochemical and histological study of the effect of antimicrobial photodynamic therapy on oral mucositis induced by 5-fluorouracil in hamsters. Photodiagnosis and Photodynamic Therapy (2015), http://dx.doi.org/10.1016/j.pdpdt.2014.12.007 ARTICLE IN PRESS +Model PDPDT-614; No. of Pages 12 Photodiagnosis and Photodynamic Therapy (2015) xxx, xxx—xxx Available online at www.sciencedirect.com ScienceDirect jou rn al hom epage: www.elsevier.com/locate/pdpdt Clinical, biochemical and histological study of the effect of antimicrobial photodynamic therapy on oral mucositis induced by 5-fluorouracil in hamsters Érika de Paula da Cruz a,1 , Luana Campos a,1 , Filipi da Silva Pereira a,1 , Gabriela Campos Magliano a,1 , Bernar Monteiro Benites a,1 , Victor Elias Arana-Chavez a,1 , Rafael Yagüe Ballester a,b,1 , Alyne Simões PhD a,* a Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil b Division of Dental Materials, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo (USP), São Paulo, SP, Brazil KEYWORDS Antimicrobial photodynamic therapy; Oral mucositis; Chemotherapy; 5-Fluorouracil; Antioxidant system Summary Oral mucositis (OM) is a debilitating side effect of chemotherapy, which can be relieved by phototherapy. Antimicrobial photodynamic therapy (aPDT) may be used for the treatment of OM, when infection is present. However, there are no studies showing that aPDT affects tissue repair process when used in the treatment of lesions caused by OM. This work aims to evaluate the effect of aPDT in healing OM induced by 5-Fluorouracil (5-FU). Two hun- dred forty-five hamsters were divided into two groups, control (C) and experimental, which were subdivided into 4 subgroups (Ch, ChP, ChL, aPDT). C group received only the vehicle of chemotherapy and anesthesia, whereas all animals of the experimental groups received anesthesia and chemotherapy agent 5-FU to induce OM. Ch group received no OM treatment; ∗ Corresponding author at: Laboratory of Oral Biology, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo. Av. Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo 05508-900, SP, Brazil. Tel.: +55 11 3019 7840; fax: +55 11 3019 7840. E-mail addresses: erika.cruz@usp.br (É.P. Cruz), luacampos@gmail.com (L. Campos), filipi.sp@hotmail.com (F.d.S. Pereira), gabrielamagliano@hotmail.com (G.C. Magliano), bernar benites@hotmail.com (B.M. Benites), vearana@usp.br (V.E. Arana-Chavez), ryb@usp.br (R.Y. Ballester), lysimoes@usp.br, lysimoes@gmail.com (A. Simões). 1 Tel.: +55 11 3019 7840; fax: +55 11 3019 7840. http://dx.doi.org/10.1016/j.pdpdt.2014.12.007 1572-1000/© 2014 Elsevier B.V. All rights reserved.