© 2018 PP House Isolaton and Molecular Characterizaton of Abiotc Stress Tolerant Plant Growth Promotng Pseudomonas spp. from Diferent Rhizospheric Soils of Telangana State, India K. Damodarachari 1* , R. Subhash Reddy 1 , S. Triveni 1 , CH. V. Durga Rani 2 and M. Sreedhar 3 1 Dept. of Agricultural Microbiology & Bioenergy, College of Agriculture, 2 Dept. of Molecular Biology & Biotechnology, Insttute of Biotechnology, 3 Quality Control Laboratory, Professor JayashankarTelangana State Agricultural University, Rajendra nagar, Hyderabad, Telengana State (500 030), India The present investgaton was carried out to isolate rhizobacteria from diferent rhizospheric soils of Telangana state, India and screened for plant growth promotng propertes such as mineral nutrient solubilizaton (P, K, Zn), IAA producton, ACC deaminase, EPS producton, biocontrol actvity and tolerance to diferent abiotc stresses such as pH, temperature, salt, drought and heavy metals in vitro conditons. Based on cultural, morphological and biochemical characterizaton, it was found that forty four were of Pseudomonas spp. Among the forty four (44) Pseudomonas isolates, twenty eight (28) isolates were showing plant growth promotng propertes. The results of diferent in vitro abiotc stress tolerance of Pseudomonas isolates, four isolates were showed growth at pH range from 4-10 (PS 1, PS 4, PS 26, PS 40). Five isolates were showed tolerance to 1.5 to 20% of NaCl concentraton (PS 4, PS 15, eight isolates were showed tolerance from 1.5 to 15% of NaCl concentraton (PS 1, PS 11, PS 18, PS 26, PS 30, PS 31, PS 32, PS 40), three isolates showed tolerance to temperature from 20 °C -50 °C (PS 11, BS 15, BS 40), nine isolates were showed tolerance to temperature from 20 °C -45 °C (PS 1, PS 4, PS 21, PS 23, PS 28, PS 30, PS 32, PS 34, PS 37), three isolates showed tolerance to water potental from -0.05 Mpa to -0.73 Mpa (PS 4, PS 15, BS 34).PS 15 was found efcient in Zn- solubilizaton with considerable other PGP propertes. It was tolerant to pH stress (4, 6), temperature stress, salt stress, drought stress and heavy metals As and Cd only. PS 1 showed good P-solubilizaton, K-solubilizaton, Zn solubilizaton with biocontrol actvity. It tolerated pH stress, temperature stress, salt stress, drought stress and tolerant to heavy metal toxicity. Performing environmental parameters for bacterial growth is also showing that bacteria can easy to survive in diferent environmental conditon. 1. Introducton Increasing crop productvity and enhancing resistance or tolerance against various stress factors has become major aim for modern agriculture (Farooq et al., 2009). In sustainable agriculture, integrated pest management is considered the most efcient strategy to manage stress causing agents, such strategy rely on combining several approaches including using resistant varietes, crop rotaton, monitoring pests, biocontrol and in severe situatons employing pestcides in an atempt to keep stress agents under control (Wegulo, 2012). Biological control forms an integral part of the IPM strategy (Landaet al., 2004). Root zone bacteria that have been found to have benefcial effects on various plants include species of the genera Arthrobacter, Azotobacter, Azospirillum, Bacillus, Enterobacter, Pseudomonas and Serrata (Gray and Smith, 2005), as well as Streptomyces spp. (Tokala et al., 2002; Dimkpa et al., 2008). Although the exact mechanisms of plant growth stmulaton remain largely speculatve, it is known that they difer between bacterial strains and most certainly depend on the various compounds released by the diferent microorganisms. The literatureis replete with reports describing the producton of the main phytohormone classes–auxins, cytokinins, gibberellins, abscisic acid and ethylene by plant growth promotng rhizobacteria (PGPR). The subject of PGPR elicited tolerance to abiotc stresses has been reviewed (Venkateswarlu et al., 2008). It has been shown that certain PGPR enhance plant stress tolerance through 1-aminocyclopropane-1- carboxylate deaminase and provide signifcant protecton to a wide range of plant species from the damage caused by various abiotc stress conditons. ACC breakdown and ethylene synthesis inhibiton by ACCdeaminase decreases the damage of various stress situatons by enhancing homeostasis in and around the plant root, especially at early stages of stress exposure (Ali et al., 2009). Pseudomonas, PGPR, pH, temperature, drought, heavy metal tolerance Keywords: Abstract Article History Artcle ID: AR1913 Received in 06 th October, 2018 Received in revised form 22 nd October, 2018 Accepted in fnal form 23 rd October, 2018 K. Damodarachari e-mail: damuagmicro2012@gmail.com Corresponding Author 611 Research Article Doi: HTTPS://DOI.ORG/10.23910/IJBSM/2018.9.5.1913 International Journal of Bio-resource and Stress Management 2018, 9(5):611-619 Stress Management