Research Article Fabrication of ECM Mimicking Bioactive Scaffold: A Regenerative Approach for MSC Mediated Applications Pavani Koka , 1,2 Yamini Chandramohan , 1 Elumalai Perumal , 3 Avinash Kavarthapu , 4 Anuradha Dhanasekaran , 5 Anusha Chandran , 6 and Krishnamoorthy Gunasekaran 7 1 Centre for Biotechnology, Anna University, Chennai, 600 025 Tamil Nadu, India 2 Bioscreen Instrumentation Pvt Ltd, Ashok Nagar, Chennai, Tamil Nadu, India 3 Center for Transdisciplinary Research, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India 4 Department of Periodontology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India 5 Cancer and Stem Cell Biology Laboratory, Centre for Biotechnology, Anna University, Chennai, 600 025 Tamil Nadu, India 6 Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India 7 Department of Medical Biochemistry, College of Health Sciences, Dambi Dollo University, P.O. Box 360, Kelam Welega Zone, Oromia Region, Ethiopia Correspondence should be addressed to Krishnamoorthy Gunasekaran; krishnapdf2011@gmail.com Received 13 July 2022; Revised 19 March 2023; Accepted 27 April 2023; Published 20 May 2023 Academic Editor: Isotta Chimenti Copyright © 2023 Pavani Koka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Biomaterials are feasible resources that aids to replace damaged structures in our bodies. The most biologically active flora is Aloe vera which has many bioactive compounds that are anti-inflammatory, antimicrobial, and have ECM mimicking protein content which helps in the healing of wounds and also acts as an ECM factor for stem cell homing and differentiation. The Aloe vera containing 10 w/v of gelatin was lyophilized. Scaffolds had sharper morphology, greater hydrophilic properties, and a Young’s modulus of 6.28 MPa and 15.9 MPa of higher tensile strength are desirable. In tissue engineering and regenerative medicine, biologically active scaffolds have been producing hopeful outcomes in both restoration and replacement, respectively. The objective of the present investigation is to test the idea that incorporating gelatin to Aloe vera scaffolds might enhance their structure, good biocompatibility, and possibly even bioactivity. The SEM picture of the composite scaffold revealed pore walls. The scaffolds had linked pores with diameters ranging from 93 to 296 μm. Aloe vera and the matrix interact well, according to the FTIR study, which could lead to a reduction in the amount of water-binding sites and a reduction in the material’s ability to absorb water. Aloe vera with 10% gelatin (AV/G) scaffold was investigated for different biological reactions of human gingival tissue mesenchymal stem cells (MSCs) in terms of cell proliferation, morphology, and cell migration. The results demonstrated the potential of the AV/G scaffold as a biomaterial that offers new insight in the field of tissue engineering. 1. Introduction The field of regenerative medicine encompasses tissue engi- neering and stem cell technology. Organ regeneration is sig- nificantly impacted by the biomedical-related research areas of tissue engineering and regenerative medicine (TERM). It incorporates clinical research along with concepts from fields like biotechnology, mechanics, cell biology, and mate- rials science. By constructing an environment that promotes cell growth and proliferation, tissue engineering is aimed at replacing and repairing damaged tissues [1] and generation of new tissue or organ which stimulation of the extracellular matrix [1, 2]. Scaffolds are made to encourage cell- biomaterial interaction, cell attachment, proliferation, and Hindawi Stem Cells International Volume 2023, Article ID 6282987, 15 pages https://doi.org/10.1155/2023/6282987