~ 797 ~ International Journal of Chemical Studies 2017; 5(3): 797-801 P-ISSN: 2349–8528 E-ISSN: 2321–4902 IJCS 2017; 5(3): 797-801 © 2017 JEZS Received: 13-03-2017 Accepted: 14-04-2017 Shri Om Verma Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India SK Pandey Department of Plant Breeding & Genetics, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India Anubha Upadhyay Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India SD Upadhyaya Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India AS Gontia Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India Correspondence Shri Om Verma Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India Biochemical changes in chickpea seed by priming and foliar application of salicylic acid and water under drought and excessive moisture stress Shri Om Verma, SK Pandey, Anubha Upadhyay, SD Upadhyaya and AS Gontia Abstract The present investigation focused on biochemical changes in JG11 and JG 14 seed by salicylic acid (SA) and Hydro-Priming with foliar application of SA and water in the drought and excessive moisture stress (EMS). Nitrogen and Protein content (%) was significantly high in JG11 (Irrigated) and JG14 (drought). Although nitrogen, protein, carbohydrate percent level was changed in JG11 and JG14 beneath drought and excessive moisture stress (EMS). However, JG11 showed high proline level (1.10 µmolg -1 ). Although JG14 expressed significant response on seed yield plant -1 under drought (8.71%) by the priming of salicylic acid (SA) with a foliar spray of 500nm SA in drought. Keywords: Chickpea seed, Nitrogen, Protein, Fiber, Carbohydrate, Proline, Drought, EMS. Introduction Chickpea (Cicer arietinum L.) is a cool season legume crop and is grown in several countries worldwide as a food source. In India during 2013-14 chickpea was cultivated in an area about 9.93 million hectares with a production about 9.53 million tones and 960 kgha -1 productivity. In Madhya Pradesh during 2013-14chickpea was cultivated in an area of about 3.16 million hectares with production 3.29 million tones and 1044kg/ha. Productivity Chickpea seeds contain on an average 23% protein, 64% total carbohydrates (47% starch, 6% soluble sugar), 5% fat, 6% crude fiber and 3% ash. High mineral content has been reported for phosphorus (340 mg100 g -1 ), calcium (190mg100g -1 ), magnesium (140mg100g -1 ), iron (7mg100g -1 ) and zinc (3 mg100g -1 ). (Anonymous 2015) [2] . According to Healthy Eating Index (HEI-2005) scores Consumers of chickpeas and/or hummus have been shown to have higher nutrient intakes of dietary fiber, polyunsaturated fatty acids, vitamin A, vitamin E, vitamin C, folate, magnesium, potassium, and iron as compared to non-consumers by Walace et al. (2016) [23] . Chickpea also improves the soil fertility due to its nitrogen-fixing ability (Maiti 2001) [13] . Mandavia et al. (2010) [14] reported that lower concentration of salicylic acid (50 ppm) was significantly increased protein content as compared to control at both vegetative and reproductive stages. Crop under stress condition changes in biochemical level along with a reduction in grain yield (Anonymous, 2013) [1] . In chickpea genotype protein percentage was recorded maximum in genotype ICC 4958 and Tyson. Whereas, the minimum was recorded in JG 315 and DCP 92-3 on the treatment of SA@1.5 mM by Patel and Hemantaranjan (2013) [18] . Water deficit plant showed reduces the growth due to influencing cell turgor, stomatal closure and enzymatic changes which are directly control by water potential by El-tayeb, (2005) [8] . Salicylic acid, as an endogenous phenolic growth regulator, adjusts the activity of antioxidant enzymes, enhances plant resistance to abiotic stresses (drought and EMS) Hayat et al (2010) [10] . Plant phenolic compounds are the most abundant and important group of defensive compounds that mediate plant defense that polyphenol level was significantly higher in plants than seeds and the highest rate of this compounds in the plant was observed with 0.5 mM SA reported by Boukraâ et al (2015) [5] The experiment focused on biochemical changes in percent viz. nitrogen, protein carbohydrate and fiber along with osmolyte (Proline) level in JG11 and JG14 chickpea genotype priming with salicylic acid (SA) and hydro-priming, foliar application SA of and water under drought and EMS.