Int.J.Curr.Microbiol.App.Sci (2018) 7(8): 139-142 139 Short Communications https://doi.org/10.20546/ijcmas.2018.708.017 Study of Complex Vertebral Malformation in Repeat Breeder and Anoestrous Cows S.B. Khade * , M.P. Sawane, V.D. Pawar, M.M. Chopade, R.D. Deshmukh and S.J. Komatwar Bombay Veterinary College, Parel, Mumbai-12, India *Corresponding author ABSTRACT Introduction Genetic disorders are genetic problem which caused by one or more abnormalities in the genome. In animals an important effect is the production of animals becomes less or loss of performance. Now a day it is possible to detect the genetic disorders on the molecular basis. There are number of autosomal recessive disorders like Complex Vertebral Malformation (CVM), Factor XI deficiency (FXID), Bovine Leucocyte Adhesion Deficiency (BLAD), Deficiency of Uridine Monophosphate synthase (DUMPS) and Bovine Citrullinamia are Holstein Friesian breed specific. CVM is autosomal recessive inherited disorder in Holstein breed which causes intra- uterine mortality leading to repeat breeding. CVM is characterised by misshapen of cervical, thoracic and lumbar vertebrae regions, ribs abnormality as well as rotation of fetlock joint. The occurrence of CVM was first time described in 2000 in Holstein calves. The molecular basis of CVM is missense mutation from G to T at nucleotide position 559 of bovine solute carrier family 35 member 3 (SLC35A3) gene. It results in V180F amino acid substitution of Golgi-resident UDP-N- acetylglucoseamine transporter enzyme which causes abnormal somitogenesis and impaired development of fetal axial skeleton. To International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 08 (2018) Journal homepage: http://www.ijcmas.com The aim of this study was to identify the Complex Vertebral Malformation (CVM) in repeat breeder and anoestrous animals in Maharashtra state region. Total 102 animals of HF crossbred, Dangi, Gaolao and Non- descript were screened from field and cattle dairy farm. CVM is a hereditary recessive disorder in Holstein cattle which have a significant economic impact on dairy cattle breeding worldwide. Fifty repeat breeder and fifty-two anoestrous animals blood DNA were extracted by traditional method. Genotyping was performed by using PCR-RFLP analysis with the help of PstI restriction enzyme. In the performed study all animals were normal for Complex Vertebral Malformation. Keywords CVM, PCR-RFLP Accepted: 04 July 2018 Available Online: 10 August 2018 Article Info