Vol. 11 | No. 2 |844 - 849 | April - June | 2018 ISSN: 0974-1496 | e-ISSN: 0976-0083 | CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in Rasayan J. Chem., 11(2), 844-849(2018) http://dx.doi.org/10.31788/RJC.2018.1122097 ANALYSIS OF COMPOUNDS AND IMMUNOSTIMULATORY PROPERTIES OF CURCUMA MANGGA RHIZOMES ON MALE MICE Yuandani 1,2,* , S. Yuliasmi 3 and D. Satria 4 1 Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, 5 Jalan Tri Dharma, USU-Kampus, Medan 20155, Indonesia, 2 Centre of Excellence for Green Chitosan and Advanced Materials, Universitas Sumatera Utara, Medan 20155, Indonesia 3 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, 5 Jalan Tri Dharma, USU-Kampus, Medan 20155, Indonesia 4 Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, 5 Jalan Tri Dharma, USU-Kampus, Medan 20155, Indonesia *E-mail: yuan_dani@yahoo.com, yuandani@usu.ac.id ABSTRACT The current study was carried out to determine the constituents in ethanol extract of Curcuma mangga rhizomes and its immunostimulatory effects of the extract on cellular and humoral immunity of male mice. Determination of constituents in the extract was conducted using Ultra Performance Liquid Chromatography. The ethanol extract of Curcuma mangga was comprised to delayed-type hypersensitivity response and haemagglutinating antibody titre tests for its immunostimulatory potential. The test animal received extract at doses of 100, 200 and 400 mg/kg BW for 7 days after sensitized by bovine Red Blood Cells. Cellular response was measured by increase of paw volume, meanwhile humoral antibody response was determined by hemagglutination method. Quercetin was determined quantitatively in the extract by a validated reversed phase Ultra Performance Liquid Chromatography method. The footpad thickness and antibody titre of male mice after treatment with ethanol extract of Curcuma mangga were significantly higher as compared to negative control (P<0.05). The extract of Curcuma mangga at a dose of 400 mg/kg BW depicted stronger immnumostimulatory activity as compared to positive control. The result indicates that the ethanol extract of Curcuma mangga shows immunostimulatory activity, emphasizing its potential to be developed as a therapeutic agent. Keywords: Curcuma mangga, immunostimulatory, delayed-type hypersensitivity, antibody titre, quercetin © RASĀYAN. All rights reserved INTRODUCTION Human immune defense consists of two main categories which include innatae (non-specific) and adaptive (specific) immunity 1 . Adaptive immunity is different from the innate response as it is specific due to the presence of an element of memory. The humoral component of acquired immunity mainly is antibody-secreting plasma cells from the proliferation of antigen-stimulated B lymphocytes. The cellular immunity (also called: cell-mediated immunity (CMI) is primarily facilitated by thymus-derived small lymphocytes (T cells), which can be divided into T helper cells (Th) and cytotoxic T lymphocytes (Tc) 2 . Th lymphocytes play an essential role in immune response because they maximize the ability of the immune response by performing several functions, such as activating phagocytes by producing interferon gamma (IFN γ), stimulating T cytotoxic cells and increase the activity of natural killer (NK) cells. In addition, T helper cells activate B cells to secrete antibodies (immunoglobulins). Thereafter, immunoglobulins circulate in the lymph and the blood streams, bind to the antigens to enhance destruction by phagocytes 3 . A number of diseases can be treated by immune response modifiers; these include immunodeficiencies and autoimmune diseases like systemic lupus erythematosus and rheumatoid