Kulkarni P K et al., Int. J. Res. Pharm. Sci., 2020, 11(1), 730-746
ORIGINAL ARTICLE
INTERNATIONAL JOURNAL OF RESEARCH IN
PHARMACEUTICAL SCIENCES
Published by JK Welfare & Pharmascope Foundation Journal Home Page: www.pharmascope.org/ijrps
Formulation and evaluation of different topical dosage forms for wound
healing properties
Dandasi Jayachandra Dev, Jayaprakash J S, Kulkarni P K
*
, Akhila A R, Namratha S Saraf
Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and
Research, JSS Medical Campus, Sri Shivarathreeshwara Nagara, Mysuru – 570015, Karnataka, India
Article History:
Received on: 04.07.2019
Revised on: 16.10.2019
Accepted on: 28.10.2019
Keywords:
wound,
β cyclodextrin,
phosphatidylcholine,
cholesterol
ABSTRACT
The aim of the present work was to compare the wound healing efϑicacy of dif-
ferent topical dosage forms such as β cyclodextrin complex gel, liposomal gel,
and ointment on the rat model. Simvastatin was used as a drug, β cyclodextrin
was used as a complexing agent to enhance solubility, L α Phosphatidylcholine
as a phospholipid, and cholesterol as a stabilizing agent. Liposomes were pre-
pared by thin-ϑilm hydration method, β cyclodextrin complexes of simvastatin
were prepared by spray drying technique, and the ointment was prepared in
simple method. Beta cyclodextrin gels and liposomal gels were prepared by
direct incorporation of spray-dried products and lyophilized liposomes into
Carbopol gel. The gel was evaluated for drug content, particle size, viscosity,
spreadability, surface morphology, in-vitro drug release studies, skin irrita-
tion study, and wound healing activity studies. FTIR and DSC studies showed
no chemical interaction between the drug and excipients. The particle size of
β cyclodextrin complexes was in the range of 0.5 μm to 2.5 μm and for lipo-
somes 163 nm to 725 nm. The in-vitro drug release was 96.7 % at the end of
the sixth hour for β cyclodextrin gel, 29.7 % at the end of the sixth hour for
liposomal gel, and 96.2 % at the end of 3 hours for ointment. Wound healing
activity studies were carried out for 21 days on albino wrister rats, a period
of epithelization, and rate of wound contraction was measured on 4, 8, 14, 16,
and 21 days. Simvastatin ointment showed a signiϑicant effect on wound heal-
ing in the rat model compared to β -cyclodextrin gel and liposomal gel. Hence,
Simvastatin ointment could be a potential dosage form for clinical utility on
wound healing.
*
Corresponding Author
Name: Kulkarni P K
Phone:
Email: pkkkulkarni@jssuni.edu.in
ISSN: 0975-7538
DOI: https://doi.org/10.26452/ijrps.v11i1.1886
Production and Hosted by
Pharmascope.org
© 2020 | All rights reserved.
INTRODUCTION
A wound may be characterized as a disturbance
of normal skin tissue structure due to physical
or thermal damage or due to the existence of an
underlying medical or physical condition and func-
tion and maybe classiϑied by etiology, position or
length. (Dhivya et al., 2015).Wound healing includes
a series of well-orchestrated biochemical and cel-
lular events leading to clear growth and regen-
eration of wounded tissue, including coagulation,
inϑlammation, the formation of granulated tissue,
epithelization, collagen synthesis, and tissue remod-
eling (Gonzalez, 2016).
There are several topical solutions available to treat
730 © International Journal of Research in Pharmaceutical Sciences