Vol.:(0123456789) 1 3 Catalysis Letters https://doi.org/10.1007/s10562-020-03362-1 Development of L‑asparaginase@hybrid Nanofowers (ASNase@HNFs) Reactor System with Enhanced Enzymatic Reusability and Stability Samir Abbas Ali Noma 1  · Burcu Somtürk Yılmaz 2  · Ahmet Ulu 1  · Nalan Özdemir 2  · Burhan Ateş 1 Received: 13 May 2020 / Accepted: 21 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract Hybrid nanofowers materials have recently received great attention in enzyme immobilization applications because of the advantages such as their large surface area, excellent stability, simple, eco-friendly, and cost-efective synthesis. In this study, L-asparaginase which is an important commercial enzyme in the medicine and food industry was selected as a model enzyme. To the best of our knowledge, this study is the frst report of designing L-asparaginase@hybrid nanofowers to enhance its enzymatic performance. L-asparaginase@hybrid nanofowers were synthesized using ASNase as an organic component and Cu(II) ion as inorganic component. They were characterized by their morphology and chemical point of view by using dif- ferent techniques. The synthesized L-asparaginase@hybrid nanofowers exhibited high residual activity at broad pH and high temperature ranges in comparison to free form. Moreover, L-asparaginase@hybrid nanofowers possessed good reusability and excellent long-time storage stability. Especially, L-asparaginase@hybrid nanofowers-3 maintained nearly 51 and 75% of its original activity, respectively, after nine consecutive catalytic cycles and storage at 30 °C for 4 weeks. The results indi- cated that these hybrid nanofowers will be promising carrier matrix for the immobilization of ASNase in biotechnological applications with improved catalytic properties. * Nalan Özdemir ozdemirn@erciyes.edu.tr * Burhan Ateş burhan.ates@inonu.edu.tr 1 Department of Chemistry, Faculty of Arts and Science, İnönü University, 44280 Malatya, Turkey 2 Department of Chemistry, Faculty of Science, Erciyes University, 38039 Kayseri, Turkey