Available online at www.worldscientificnews.com ( Received 24 April 2019; Accepted 11 May 2019; Date of Publication 13 August 2019 ) WSN 134(2) (2019) 74-85 EISSN 2392-2192 The Effect of Garlic Extract Addition on Tilapia Skin Gelatin Based Edible Coating Towards Antimicrobial Properties and Fish Meatball’s Shelf Life Ahmad Reynaldi*, Iis Rostini, Eddy Afrianto, Emma Rochima Faculty of Fisheries and Marine Sciences Universitas Padjadjaran Jl. Raya Jatinangor KM. 21 Sumedang, Indonesia *E-mail address: sephiroth.ahmad@gmail.com ABSTRACT The purpose of this research is to determine the best concentration and effect of garlic extract incorporated on tilapia skin gelatin-based edible coating to preserve and extend the shelf life of fish meatball at cold storage. This research was conducted from May 2018 until October 2018 on Fisheries Product Processing Laboratory of The Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The garlic extract conducted with maceration method with ethanol 96% was performed at Organic Chemical Laboratory FMIPA Universitas Padjadjaran, and for TPC test, inhibition test of edible coating Tilapia’s fish-skin with garlic extract was conducted in Microbiology and Molecular Biotechnology Laboratory of Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. The method used is experimental method with 5 treatments which is meatballs without edible coating. The addition of garlic extract was done with various concentration at 0%, 1%, 1.5%, 2%. The observations were Total Plate Count (TPC) test, inhibition zone test against Pseudomonas aeruginosa, and pH value. The results showed that the best treatment is 2% garlic extract with TPC value 1.1×10 5 cfu/g during 14 days of storage and concluded that it could extend the shelf life of meatball until 14 days, pH value was 7.03 and inhibition zone against P. aeruginosa was 7.01 mm. The addition of garlic extract on tilapia skin gelatin-based edible coating could extend the shelf life of fish meatballs until 14 days on cold storage. Keywords: edible coating, extract, garlic, pH values, shelf life, tilapia gelatin, TPC, Pseudomonas aeruginosa, Staphyllococcus aureus, Salmonella typhimurium, E. coli