Citation: Rashid A. Study on Pressure Overload-Induced Cardiac Hypertrophy Model by Applying Transverse Aortic Constriction and O-ring Ascending Aortic Banding Technique: A Comparative Analysis. Ann Surg Perioper Care. 2020; 5(1): 1043. Ann Surg Perioper Care - Volume 5 Issue 1 - 2020 Submit your Manuscript | www.austinpublishinggroup.com Rashid. © All rights are reserved Annals of Surgery and Perioperative Care Open Access Abstract Left-ventricular cardiac hypertrophy is a well-established precursor of heart failure with the ominous prognosis. To develop the surgical procedure that can easily and effciently replicate this condition is too critical, and is signifcantly important for the better understanding of the pathophysiological mechanisms associated with this vascular disease. Constriction of the aorta in mice is a highly accepted method for the creation of pressure overload-induced cardiac hypertrophy model. The two microsurgical procedures available for the successful development of the disease model are aortic constriction at the level of transverse aorta and o-ring aortic banding using the ascending aorta. The transverse aortic constriction has many advantages over the other available technique which is signifcantly highlighted in this paper. Using transverse aorta, though the degree of constriction is widely dependent on the diameter of the gauge needle. Our main purpose is to establish a comparison between these two surgical procedures to highlight the highly reproducible and precise method to study the mechanism of the disease progression as well as cardiac remodeling. leads to the development of this condition, and it also mimics the similar condition in humans which results in the chronic systemic hypertension or aortic stenosis [4]. Tere are three diferent positions for the creation of aortic constriction, these are descending aorta, ascending aorta and transverse aorta [6]. Among this, one of the most common surgical models of heart failure and pressure overload-induced cardiac hypertrophy, is the constriction at the level of the transverse aorta which is widely known as Transverse Aortic Constriction (TAC). Tus, for the successful creation of pressure overload in the lef ventricle, constriction on a permanent basis around the transverse part of the aorta must be achieved which further limits the blood outfow towards the lef ventricle [3-5]. TAC can produce cardiac diastolic and systolic dysfunction and long-term progression to heart failure in addition to cardiac remodeling efects such as fbrosis and hypertrophy [4]. Te other method used for the development of the disease in mice is the ascending aortic constriction through o-rings with fxed inner diameters which is known as O-Ring Aortic Banding (ORAB). Hence, our aim is to compare two most important aortic constriction methods to highlight the highly reproducible and precise method to study the mechanism of the disease progression as well as cardiac remodeling. Methodology Latest reports indicate a substantial association between ethnic backgrounds or race and the hypertrophy development, which Introduction Vascular diseases are the major cause of mortality and morbidity and associated with high societal costs [1]. Te understanding of the pathological mechanism’s underlying cardiovascular remodeling; although critical but essential for the development of innovative therapies for vascular diseases [2]. At the frst phase of this pathological cardiac remodeling process, the heart undergoes hypertrophic growth through each myocytes enlargement which does not afect the normal function of the heart. Te main purpose of this remodeling is to enhance the cardiac pump function [3]. However, the continued stress would lead to the fbrosis and enlargement of the heart chamber. Reproducibility is expected in research related to heart diseases, and validity, and accuracy, is of great concern [2]. It is well established that related conduits control the vasculature and heart development in mice and humans. Terefore, preclinical studies continue to rely heavily on mouse models and thus these models are useful both for the development of drugs with the study of mechanism [4]. Since the heart has a negligible capacity of regeneration, most form of cardiac injury ultimately results in fbrosis development [2-4]. Te further development of novel and innovative techniques would also highlight major steps forward in the modeling of vascular diseases. In cardiovascular research, surgically induced models of heart failure are widely used. Constriction of the aorta is a well-recognized method for lef ventricular hypertrophy induction in mice [5]. As in the lef ventricular chamber, increased blood pressure ultimately Review Article Study on Pressure Overload-Induced Cardiac Hypertrophy Model by Applying Transverse Aortic Constriction and O-ring Ascending Aortic Banding Technique: A Comparative Analysis Anas Rashid* School of Medicine (Vascular Surgery), University of Turin, Turin, Italy *Corresponding author: Anas Rashid (Vascular Surgery), Graduate School of Medical Science and Engineering, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea Received: June 27, 2020; Accepted: October 17, 2020; Published: October 24, 2020