Mediterranean Journal of Basic and Applied Sciences (MJBAS) Volume 7, Issue 2, Pages 90-96, April-June 2023 ISSN: 2581-5059 90 Renal Biochemical Marker Changes in Restorative Activities of Curcuma longa on Sildenafil Induced Nephrotoxicity Among Male Albino Rats Khisa Wanjala Allan 1* , Marera Oduor Domnic 1 & Adero Walter 1 1 Department of Human Anatomy, School of Medicine, Maseno University, Kisumu, Kenya. Corresponding Author (Khisa Wanjala Allan) Email: allanwanjala345@gmail.com* DOI: https://doi.org/10.46382/MJBAS.2023.7211 Copyright: © 2023 Khisa Wanjala Allan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Article Received: 05 April 2023 Article Accepted: 26 May 2023 Article Published: 05 June 2023 ░ Introduction Kidney biomarkers are the chemicals used to determine any decrease in kidney function and estimate both severity and nature of kidney injury. These biomarkers include lipids, proteins, genes, metabolites and cells present on urinalysis. Drug induced nephrotoxicity remains the leading cause of acute kidney injury. Majority of the population affected being from industrialized countries and this is attributed to the excess environmental pollution witnessed, lifestyle changes that has led to the development of hypertension and diabetes mellitus. Therefore, there is a high need of increase sensitivity and specificity of different tools, need to combine different biomarkers so as to increase the diagnostic accuracy (Wasung et al., 2015) used to detect drug induced nephrotoxicity as a cause of acute kidney injury at early stages. The emerging new biochemical markers include: kidney injury molecule 1 and 2, Cystatin C and FGF-23 (Wasung et al., 2015). Serum creatine remains the major kidney biomarker however, it is associated with challenges like patients with low muscle bulky and fluid overload it may be hard for you to use Serum creatinine as biomarker therefore, there is Sildenafil has been associated with nephrotoxicity because of increase in rate of reports of patients developing acute kidney injury upon its intake. It has cytotoxic effects as it’s known to damage cell membrane. It also increases levels of lactate, calcium thus causing calcification and necrosis of tubules. In human beings’ sildenafil causes increased blood urea nitrogen and serum creatinine levels. This is based on the management of 67-year-old ABSTRACT Background: Nephrotoxicity is the rapid deterioration of kidney function due to altered histoarchitecture and drug clearance characterized by increased levels of renal biochemical markers. Sildenafil is among the drugs postulated to cause nephrotoxicity if taken over a long period of time or as an overdose as it interferes with the kidney histoarchitecture. Curcuma longa is a traditional herbal plant which is used in diet, treatment of diabetes, dermatological conditions and renal diseases. Objective: The aim of this study was to evaluate renal biochemical marker changes in restorative activities of Curcuma longa on sildenafil induced nephrotoxicity. Methodology: A total of 25 male albino rats of species rattus norvegiccus were used in this experiment. 5 rats received water and feeds, 20 received 1μg/gmbwt Sildenafil for 15 days from these 5 rats were sacrificed four hours post last dose. The remaining 15 rats were treated with Curcuma longa at calculated doses. Animals were sacrificed in humane end points, blood collected through cardiac puncture stored in sample bottle and centrifuged to harvest serum. Results: It was observed that blood urea and creatinine levels in Sildenafil-induced nephrotoxicity group significantly increased as compared to negative control group and reduced significantly in medium and high dose Curcuma longa groups. Conclusion: Based on this study it can be concluded that medium and high dose Curcuma longa reduces blood urea and nitrogen and creatinine levels when used in the assessment of restorative effects of Curcuma longa on Sildenafil induced nephrotoxicity among male albino rats. Keywords: Biomarkers; Histoarchitecture; Kidney; Nephrotoxicity; Restoration.