Plant Protection, 04 (01) 2020. 35-42 DOI: 10.33804/pp.004.01.3209 35 Available Online at EScience Press Plant Protection ISSN: 2617-1287 (Online), 2617-1279 (Print) http://esciencepress.net/journals/PP MANAGEMENT OF AEDES AEGYPTI USING GREEN SILVER NANOPARTICLES AND BOTANICAL EXTRACTS Hassan Saeed 1 , Muhammad Tariq 1 , Asim Gulzar 1 , Zia-ur-Rehman Mashwani 2 , Muhammad Safian Bajwa 1 1 Department of Entomology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. 2 Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan. A R T I C L E I N F O A B S T R A C T Article history Received: 31 st January, 2020 Revised: 19 th March, 2020 Accepted: 22 nd March, 2020 The excessive use of insecticides has led to development of resistance in Aedes aegypti and negative impact on environment and non-target organisms. To overcome these problems, emphasis is being laid for alternatives, therefore, in the present study, the toxicity of eight plant extracts and their green synthesized nanoparticles were evaluated against A. aegypti. Clove extracts caused the maximum mortalities of 92% and 90% of 3 rd and 4 th instar larvae of A. aegypti followed by ginger causing 90% and 78% mortalities respectively. On the other hand, the minimum mortalities of these larvae were caused by neem and garlic extracts. In case of green silver nanoparticles, the maximum mortalities of 3 rd and 4 th instar larvae of A. aegypti were caused by clove followed by ginger while the minimum mortalities were caused by nanoparticles of datura followed by garlic. All the green silver nanoparticles caused mortalities of both the instars of the mosquito above 80% with few exceptions. Datura extracts showed the minimum LC50 values after 72 hours of application followed by neem against the 3 rd and 4 th instar larvae of A. aegypti. The highest LC50 value was observed in case of ginger followed by clove and eucalyptus. In case of silver nanoparticles, the minimum LC50 values after 72 hours were recorded in case of datura, neem and garlic while the values were the maximum in case of clove and ginger. The LC50 values decreased with the passage of time. Keywords Yellow fever mosquito Botanical extracts Green synthesized silver nanoparticles LC50 Toxicity Corresponding Author: Hassan Saeed Email: hassan.saeed909@gmail.com © 2020 EScience Press. All rights reserved. INTRODUCTION The yellow fever mosquito, Aedes aegypti (Diptera: Culicidae) is a vector of several arboviruses such as chikungunya virus (Leparc-Goffart et al., 2014), zika virus (Gutiérrez-Bugallo et al., 2019) and dengue virus (Morrison et al., 2019). Dengue is the most important mosquito borne disease (Li et al., 2019) and over 390 million humans are effected by dengue virus per year in the world (WHO, 2018). Dengue and zika fever do not have any specific medication as a cure, so the most important measure is to control the mosquitoes of these diseases. These control measures include the use of synthetic insecticides (Paul et al., 2006), essential oils (Pandiyan et al., 2019) and microorganism based insecticides (Vasantha-Srinivasan et al., 2019) against mosquitoes but the chemical based insecticides are