 Corresponding author: Afnan KV Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0. Formulation and evaluation of herbal nanosuspension of Scoparia dulcis Afnan KV 1, * , Jamsheer KA 2 , Safa Muhammed P 1 and Raheena KA 1 1 Department of pharmaceutics, Al Shifa College of Pharmacy, Poonthavanam PO, Perinthalmanna, Kerala, India- 679325. 2 Al Shifa College of Pharmacy, Poonthavanam PO, Perinthalmanna, Kerala, India- 679325. World Journal of Biology Pharmacy and Health Sciences, 2023, 13(01), 339–343 Publication history: Received on 16 December 2022; revised on 19 January 2023; accepted on 22 January 2023 Article DOI: https://doi.org/10.30574/wjbphs.2023.13.1.0055 Abstract Background: The aim of this study is to formulate nanosuspension of Scoparia dulcis. This plant species is mainly used as an analgesic, antipyretic, and urinary issues as well as stomach disorders, kidney stones, hypertension, diabetes, and inflammation. In this study alcoholic extracts of Scoparia dulcis are used. The ionic gelation method is the most suitable method to formulate polyherbal nanosuspension. Methods: Herbal nanosuspension was prepared by the emulsion ionic gelation method. Chitosan (CS) is a polymer, and Sodium tripolyphosphate (STPP) is an inorganic compound. Polyethylene glycol (PEG) is used as a stabilizer. Result: The herbal nanosuspension formulations were evaluated for pH, viscosity, and stability. Conclusion: Using optimal nanoparticles herbal nanosuspension is formulated. The F4 formulation has proper viscosity and stability. Keywords: Scoparia dulcis; Herbal nanosuspension; Ionic gelation method; Chitosan; Polyethylene glycol 1. Introduction Many illnesses and disorders can benefit from using medicinal herbs as an alternative. They are inexpensive and frequently provide fewer negative effects. Even when used in conjunction with other medications, herbal remedies can still have negative impacts on health [1]. According to recent estimates from the World Health Organization (WHO), 80% of people worldwide rely on herbal remedies for some part of their basic medical needs. Around 21,000 plant species have the potential to be used as medicinal plants, according to the WHO [2,3]. The use of medicinal herbs is seen to be quite safe because there are rarely any negative side effects. The major benefit is that these treatments work in harmony with nature. The usage of herbal remedies can benefit people of all ages and genders, which is a key fact [4-6]. Solid colloidal particles known as nanoparticles have sizes between 10 and 1000 nm. Drugs can be linked to a nanoparticle matrix, dissolved in it, or trapped inside of it. Drugs may potentially be absorbed on the surface of these systems since they have such high surface areas. Drugs, proteins, and DNA are efficiently transported to target cells and organs via polymer-based nanoparticles. Their nanoscale size encourages stability in the bloodstream and efficient diffusion through cell membranes [5-7].