P.1.a. Basic and clinical neuroscience − Genetics and epigenetics S177 P.1.a.036 Subclinical psychotic experiences in healthy adults: relationship between genetic variants of FKBP5 gene, neuroticism and childhood trauma M. Mihaljevic 1° , T. Pekmezovic 2 , S. Andric 3 , T. Mirjanic 4 , I. Novakovic 5 , N. Maric 6 1 Clinic for Psychiatry, Clinical Center of Serbia, Research and Early Interventions in Psychiatry, Belgrade, Serbia; 2 Institute for Epidemiology, Faculty of Medicine, Belgrade, Serbia; 3 School of Medicine, University of Belgrade, Belgrade, Serbia; 4 Special Hospital for Psychiatric Disorders Kovin, Special Hospital for Psychiatric Disorders, Kovin, Serbia; 5 Institute for Human Genetics, Faculty of Medicine, Belgrade, Serbia; 6 Clinic for Psychiatry, Clinical Center of Serbia, Faculty of Medicine, Belgrade, Serbia Objective: Affective dysregulation may be the one of po- tential mechanisms for heterogenic etiology of psychosis [1]. Hypothalamic–pituitary–adrenocortical (HPA) axis is the main biological system involved in stress response and affective reg- ulation. Glucocorticoid signaling plays a pivotal role in the link between trauma-induced HPA axis alteration and psychosis. Func- tional single nucleotide polymorphisms (SNPs) in FK506-binding protein 51 gene (FKBP5), which modulates the glucocorticoid receptor sensitivity, and childhood trauma (CT) are associated with subclinical psychotic symptoms [2]. CT is influencing affective pathway with proneness to negative affectivity and its proposed measure - neuroticism (N) [3]. Higher neuroticism is associated with higher levels of both clinical and subclinical psychotic expe- riences [4]. We investigated relationship between CT, N and SNPs of the FKBP5 gene in predicting subclinical psychotic experiences (P) in healthy adults. Methods: Genetically homogeneous sample of ninety eight (98) healthy participants from Serbia (44% male, age 29.1±6.5 years, mean IQ 106.2±16.1) were genotyped for rs9296158, rs3800373 and rs9470080. All genotypes were tested for deviation from Hardy-Weinberg equilibrium. Evaluation of N and CT was performed by using the Neuroticism scale from Eysenck Person- ality Questionnaire (EPQ) and Childhood Trauma Questionnaire (CTQ). Level of subclinical psychotic experience was measured by Community Assessment of Psychic Experiences (CAPE) using the subscale for positive symptoms. Hierarchical multiple regression analysis was conducted to identify predictors of P and N. Predictor variables were separated into four blocks (models): age, gender and IQ were in the first model; N (psychological factor) was in the second model (only for P); CT (social factor) was in the third model; and SNPs (biological factors) were in the fourth model. The interaction between CT and SNPs was assessed by univariate general linear model. The data collection was performed in collaboration with EU-GEI research network [5]. Results: The hierarchical regression analysis showed that only N and CT were significant predictors of P (p=.005, p=.001). N ex- plained 37% of the variance of subclinical expression of psychosis. Addition of CT explained 49% of the variance. Rs9296158, rs9470080, and CT were significant predictors for N (respectively p=.054, p=.043, p=.001) and explained 43% of the variance of N. All three SNPs (rs9296158, rs3800373, rs9470080) showed significant interaction with CT in the model for P as an outcome variable (respectively F=10.22, p=.002; F=10.37, p=.002; F=6.40, p=.01). Age, gender and IQ had no significance in the model. Conclusions: This is the first study to our knowledge that used bio-psycho-social approach for subclinical psychotic experiences. We showed relationship between biological factors (SNPs for FKBP5 gene) and psychological factor (N), and also confirmed significant impact of biological and social factor (CT) interaction related to P as an outcome. Our study gave further evidence that functional SNPs of FKBP5 could be involved in etiology of psychosis as a part of stress related pathway. We suggest that FKBP5 gene should be considered as a marker for stress sensitiv- ity endophenotype across diagnostic boundaries. Understanding the relationship among bio-psycho-social factors can improve identification of high risk individuals and contribute for prevention to psychosis. References [1] van Rossum I, Dominguez MD, Lieb R, Wittchen HU, van Os J., 2011. Affective dysregulation and reality distortion: a 10-year prospective study of their association and clinical relevance. Schizophr Bull. 37, 561-71. [2] Collip D, Myin-Germeys I, Wichers M, Jacobs N, Derom C, Thiery E, Lataster T, Simons C, Delespaul P, Marcelis M, van Os J, van Winkel R., 2013. FKBP5 as a possible moderator of the psychosis-inducing effects of childhood trauma. Br J Psychiatry. 202, 61-8. [3] Ormel J, Bastiaansen A, Riese H, Bos EH, Servaas M, Ellenbogen M, Rosmalen JG, Aleman A., 2013. The biological and psychological basis of neuroticism: current status and future directions. Neurosci Biobehav Rev. 37, 59-72. [4] Boyette LL, Korver-Nieberg N, Verweij K, Meijer C, Dingemans P, Cahn W, de Haan L; GROUP., 2013. Associations between the Five- Factor Model personality traits and psychotic experiences in patients with psychotic disorders, their siblings and controls. Psychiatry Res. 15, 491-7. [5] European Network of National Networks studying Gene-Environment Interactions in Schizophrenia (EU-GEI), (group authorship), 2014. Identifying Gene-Environment Interactions in Schizophrenia: Contem- porary Challenges for Integrated, Large-scale Investigations. Schizophr Bull. 40, 729-36. P.1.a.037 Compulsive behaviour in adolescent patients with anorexia nervosa N. Israelyan 1° 1 Yerevan State Medical University after M. Heratsi, Department of Psychiatry, Yerevan, Armenia, Republic of Purpose of the study: According to contemporary literature data obsessive-compulsive disorders (OCD) are often accompanied by body dismorfic disorder and anorexia nervosa [1,2]. This comorbidity complicates the clinical feature and course of OCD affecting diagnostic process. The study of these overlap syndromes is significantly important in adolescents connected to age related changes and complications in clinical feature [3]. The aim of current study is to examine the relationship of anorexia nervosa and compulsive symptoms in adolescents. For this reason we have made dynamic clinical study of 45 patients (40 girls and 5 boys; age 14-18 years old) with anorexia nervosa during the period of 2 years. Methods: Obsessive compulsive symptoms have been esti- mated by Yale-Brown Obsessive Compulsive Scale (YBOCS). Clinical assessment includes usage of scale for semi structured clinical interview. The studied cases were evaluated according to ICD 10 criteria for obsessive compulsive disorder and anorexia nervosa. Summary of results: Among 45 patients with anorexia nervosa 18 had OCD symptoms. Among patients with obsessive compul- sive symptoms were identified 3 groups. In the first group (4 patients; 22.2%) compulsive behavior of anorectic patients was expressed by compulsive checking of