www.stke.org/cgi/content/full/sigtrans;2003/174/pl6 Page 1 INTRODUCTION MATERIALS Fly Strains Preparation Chamber Dissection Stimulation EQUIPMENT Microscope Illumination CCD Camera Filter Settings and Beam-Splitter Device Computer Control of the CCD Camera and Data Acquisition RECIPES INSTRUCTIONS Experimental Preparation Data Acquisition Data Evaluation TROUBLESHOOTING RELATED TECHNIQUES NOTES AND REMARKS Data Presentation REFERENCES In Vivo Calcium Imaging of Brain Activity in Drosophila by Transgenic Cameleon Expression André Fiala* and Thomas Spall (Published 18 March 2003) P ROTOCOL Julius-Maximilians-Universität Würzburg, Theodor Boveri-Institut für Biowissenschaften, Lehrstuhl für Genetik und Neurobiologie, Biozen- trum, Am Hubland 97074, Würzburg, Germany *Corresponding author. Telephone: +49-931-888-4482, fax: +49-931-888-4452, e-mail: afiala@biozentrum.uni-wuerzburg.de.