ISSN: 2319 – 8753 International Journal of Innovative Research in Science, Engineering and Technology Vol. 1, Issue 2, December 2012 Copyright to IJIRSET www.ijirset.com 226 Isolation of Pseudomonas Syringe from Hazelnut Trees in West Part of Mazandaran Province S. E Mahdavian 1 , N Hasanzadeh 2 Natural Resources and Agricultural Research center of Mazandaran, Iran 1 and Islamic Azad University, Science and Research Branch, Tehran,Iran 2 ABSTRACT: Symptoms such as yellowing, wilting and vascular discoloration in hazelnut orchards (Corylus avellanae) observed in the Dohezar region of Tonekabon. After sampling taken from plants which have mentioned symptoms and culture on nutrient agar containing sucrose, positive levan Pseudomonases were isolated. The isolates produce Green Fluorescent Pigments in King,s B Medium. These isolates didn’t grow in Medium containing %5 salt and %1 TTC and unable to create soft rot on the potato. The isolates were negative in terms of oxidase and pectinase but they were positive in terms of make an ice core and catalase. Isolated bacteria showed hypersensitive reaction on tobacco leaves but they unable to hydrolysis gelatin, starch tween 80 and create soft rot on potato. Obtained strains used casein, trehalose, glucose, L – glutamate and aspartate but they unable to use M- tartarate, L-lysine and beta-alanine as a carbon source. Isolates with inoculate suspension of bacteria on 2 years seedlings of hazelnut was Pathogenic and they had ablility to produce Syringomycin in biological assessment on Bacillus subtilis and Geotricum candidum in PDA Medium. Isolates were identified as a P.syringe based on obtained results of physiological and biological tests. This is the first report from P.syringe bacteria as causal agent of hazelnut decline in Iran. Key words: bacteria, canker, hazelnut, Pseudomonas syringe I.INTRODUCTION Hazelnut (Corylus avellanae) is deciduous shrub and a Monoecious of Alder family. Common hazelnut originated from regions such as the south of Europe and Asia Minor and the planted of it was prevalent in Europe from the past. Provinces such as Urmia, Gorgan, Gillan and Mazandaran are the important regions for grow the hazelnut in Iran (Khoshkhuy et al., 1991). Bacterial canker is a very dangerous disease for hazelnut. This disease cause to yellowing, wilting Falling leaves and buds and ultimately the death of infected trees which make an irrecoverable damage for Orchardist in epidemic years. During five years, over 1800 hazelnut trees lost just in the garden located in Rom due to this disease (Scortichini and Tropiano, 1994). Hazelnut Bacterial canker reported from Greek in 1979 (Psallidas and Panagopoulos, 1979). During 1995, 1996 and 1997, P. syringe pv. avallanae on infected hazelnut trees was isolated and reported from different regions of Italy (Scortichini and Morone, 1997). Disease occurrence and wilter of hazelnut branches due to Pseudomonas syringe pv. coryli was reported during 2005 and 2007 from Italy(Cirvilleri et al., 2007; Scortichini et al., 2005) and from Germany in 2006 (Poschenrieder et al., 2006). Taxonomy of bacterial canker disease of hazelnut studied in 1984 (Psallidas, 1984). Reclassification of pseudomonas syringe pv.avellanae as pseudomonas avellanae (Spec.nov) reported by Psallidas and Vrijer (Psallidas and Vrijer, 1996). Molecular analysis performed about pseudomonas syringe pv. coryli by lortil et al (Loretil et al.,2008). Current report was the result of studies about P. syringe which its existence reported first in Iran. II.MATERIAL AND METHODS Survey and Sampling In taken Survey from Hazelnut orchards of Tonekabon Township, suspicious trees were observed which have yellow, wilting and decline leaves (fig. 1).