Hassana et al. Ind. J. Pure App. Biosci. (2019) 7(4), 31-37 ISSN: 2582 – 2845 Copyright © July-Aug., 2019; IJPAB 31 Molecular Identification of Hydrocarbon Degrading Bacteria Isolated from Contaminated Soil of Automobile Mechanic Workshop in Lapai, Niger State Abubakar Hassana 1* , Balogu Tochukwu Vincent 2 , Ogar Deborah Ushuji 1 , Ndatsu Yakubu 1 , Usman Hamza Boko 1 and Mayaki Fatima Gogo 1 1 Department of Biochemistry, Ibrahim Badamasi Babangida University, Lapai, Nigeria 2 Department of Microbiology, Ibrahim Badamasi Babangida University, Lapai, Nigeria *Corresponding Author E-mail: ahassana@ibbu.edu.ng Received: 24.06.2019 | Revised: 30.07.2019 | Accepted: 5.08.2019 INTRODUCTION Environmental contamination by hydrocarbon is becoming rampant possibly as a result of the substantial dependence on petroleum as the main source of energy across the globe, population expansion, rapid industrial development and total negligence for the environmental wellbeing (Abioye, 2011). Petroleum hydrocarbons are the organic contaminants of key interest because of their complex structure, widespread, toxicity and persistence. Available online at www.ijpab.com DOI: http://dx.doi.org/10.18782/2320-7051.7588 ISSN: 2582 – 2845 Ind. J. Pure App. Biosci. (2019) 7(4), 31-37 ABSTRACT Hydrocarbon contamination is one of the central environmental problems today as a result of petrochemical industries’ related activities. Petroleum hydrocarbons are the organic contaminants of key interest because of their complex structure, wide spread, toxicity and persistence. Accumulation of these pollutants in plants and animal tissues has been reported to cause death or mutation. Hence this study focused on molecular identification of hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil of automobile workshop in Lapai, Niger State, Nigeria. Isolation was carried out on Mineral Salt Media (MSM) containing 1% used engine oil as carbon source. The isolates hydrocarbon degradative activities were determined using turbidimetric analysis. The isolate with fastest growth was characterized based on morphology, biochemical test and subsequent molecular identification using 16S rRNA gene sequence analysis. Six bacteria strains; FS1, FS2, FS3, ES1, ES2 and ES3 that utilized 1% used engine oil as sole carbon and energy were isolated. Bacteria ES2 effectively utilized the used engine oil with the highest Optical density (OD) of 0.96 at 600 nm on the 10 th day. Result from the biochemical test, blasting and phylogenetic analysis showed that bacteria ES2 belongs to Lysinibacillus specie with 89% maximum identity to this specie. These bacteria can be used to effectively bio remediate hydrocarbon polluted environment. Keywords: Hydrocarbon, Contaminated soil, Molecular Identification, Lysinibacillus. Research Article Cite this article: Hassana, A., Vincent, B.T., Ushuji, O.D., Yakubu, N., Boko, U.H., & Gogo, M.F. (2019). Molecular Identification of Hydrocarbon Degrading Bacteria Isolated from Contaminated Soil of Automobile Mechanic Workshop in Lapai, Niger State., Ind. J. Pure App. Biosci. 7(4), 31-37. doi: http://dx.doi.org/10.18782/2320-7051.7588