_____________________________________________________________________________________________________ *Corresponding author: E-mail: hyahaya.mls@buk.edu.ng; Asian Journal of Research in Infectious Diseases 4(3): 5-17, 2020; Article no.AJRID.58961 ISSN: 2582-3221 Lassa Virus Diagnostic Platforms: Limitations and Prospects Yahaya Hassan 1,2* , Abdulhadi Sale Kumurya 1 , Ibrahim Aminu 1 , Sanusi Rahinatu Sharfadi 1 and Abdullahi Alhassan Sharif 2,3 1 Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bayero University Kano, Nigeria. 2 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Selangor, Malaysia. 3 Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, Bayero University Kano, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJRID/2020/v4i330147 Editor(s): (1) Dr. Bobby Joseph, St. John’s Medical College, India. Reviewers: (1) J. Dhivya, Kumaraguru College of Technology, India. (2) Devajani Deka, Central Agricultural University, India. (3) Mohammad J. Al-Jassani, Al Karkh University of Science, Iraq. Complete Peer review History: http://www.sdiarticle4.com/review-history/58961 Received 04 May 2020 Accepted 10 July 2020 Published 22 July 2020 ABSTRACT Background: Lassa virus (LASV) is the cause of lassa fever (LF) belonging to the Arenaviridae family. Clinical diagnosis is often difficult because of symptoms commonality with other infectious diseases. Early and rapid diagnosis is critical for therapy initiation and LF transmission prevention and control. Aims: This review aims to highlight current diagnostic platforms and prospects of new emerging sensitive platforms. Methodology: Available published articles on LASV diagnostics with a focus on current methods: virus culture, enzyme-linked immunosorbent assay (ELISA), reverse transcriptase-polymerase chain reaction (RT – PCR) and rapid diagnostic tests (RDT) were reviewed based on their performances and limitations. Prospects of new diagnostic platforms: mobile health, microfluidic, Review Article