Nanomedicine (Lond.) (Epub ahead of print) ISSN 1743-5889
part of
Research Article
10.2217/nnm-2017-0040 © 2017 Future Medicine Ltd
Aim: The development of surface-tailored quercetin (QCT)–salicylic acid (SA) physical
complex for effective treatment of skin carcinoma. Materials & methods: QCT–SA
nanomixed micelles were prepared by the self-assembly method employing the
Quality by Design (QbD) approach and evaluated for various in vitro, ex vivo and
in vivo parameters. Results: The optimized formulation showed enhanced percent
permeation (Q
24
), in other words, 78.12 ± 0.47, improved in vitro cellular uptake and
annexin-V–apoptosis assay exhibited 60.86% cell death. The 7,12-dimethylbenz(a)
anthracene and histopathology protocol revealed the improved antineoplastic action
of QCT. The dermatokinetic profile showed the maximum drug concentration (6 h),
in other words, 416.02 ± 26 μg/cm
2
in epidermis and 103.65 ± 12 μg/cm
2
in dermis.
Conclusion: The overall performance ratified the safety and efficacy of optimized
nanomixed micelless of QCT with SA in a synergistic manner.
First draft submitted: 30 January 2017; Accepted for publication: 3 April 2017;
Published online: 19 May 2017
Keywords: apoptosis•histopathology•nanomicelles•physicalcomplex•skincancer
Surface-tailored nanomixed micelles
containing quercetin–salicylic acid physical
complex for enhanced cellular and in vivo
activities: a quality by design perspective
Premjeet Singh
Sandhu***
,1,2,3
, Rajendra
Kumar
1,4
, OP Katare**
,2,5
&
Bhupinder Singh*
,1,2,3
1
UGC-CentreofExcellencein
ApplicationsofNanomaterials,
Nanoparticles&Nanocomposites,Panjab
University,Chandigarh160014,India
2
DivisionofPharmaceutics,University
InstituteofPharmaceuticalSciences,
PanjabUniversity,Chandigarh160014,
India
3
UGC-CentreofAdvancedStudies(CAS),
PanjabUniversity,Chandigarh160014,
India
4
PostGraduateInstituteofMedical
Research&Education(PGIMER)Hospital,
Chandigarh160015,India
5
ResearchPromotionCell,Panjab
University,Chandigarh160014,India
*Authorforcorrespondence:
bsbhoop@pu.ac.in
bsbhoop@gmail.com
**Authorforcorrespondence:
dropkatare@yahoo.com
director_research@pu.ac.in
***Authorforcorrespondence:
pssandhu.rgtu@gmail.com
premjeetsandhu27@gmail.com
QbD-optimization
Stirring for 5 min at 2000 RPM
Incorporation in secondary gel
QCT SA complex
Nano micelles
Aqueous phase
Water + TritonX-100
Organic phase
PL + T80 + EtOH
+
QCT + SCA
Self assembly
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