TEKNIKA: JURNAL SAINS DAN TEKNOLOGI VOL 18 NO 02 (2022) 102–110 TEKNIKA: JURNAL SAINS DAN TEKNOLOGI Homepage jurnal: http://jurnal.untirta.ac.id/index.php/ju-tek/ Herayati Herayati a,b , Agus Rochmat a,b,1 , Lia Nurliana c , Meri Yulvianti b , Yeyen Maryani a,b , Ahmad Hariri d , Suaedah Suaedah e a Center of Excellence for Local Food Innovation University of Sultan Ageng Tirtayasa b Department of Chemical Enginering, Universitas Sultan Ageng Tirtayasa Banten c Departemen Kebidanan, Universitas Faletehan Banten d Departemen Radiologi dan Radioterapi, STIKES Pertamedika Jakarta e MTsN 4 Indramayu, Jawa Barat 1 Corresponding author: agus_rochmat@untirta.ac.id A R T I C L E I N F O Article history: Submitted 09 August 2022 Received 19 September 2022 Received in revised form 05 October 2022 Accepted 25 October 2022 Available online on 30 November 2022 Keywords: Crescentia cujete L Fruit, Docking in Silico, Glutathion perxidase, Chatepsin K. Kata kunci: Crescentia cujete L Fruit, Docking in Silico, Glutathion perxidase, Chatepsin K. A B S T R A C T Osteoporosis is a metabolic bone disease characterized by a decrease in bone mass, caused by a reduction in bone matrix and minerals accompanied by microarchitectural deterioration of bone tissue. Factors such as hormone production, the aging process, and the formation of free radicals due to oxidative stress by Reactive Oxygen Species (ROS) can stimulate bone resorption. The content of secondary metabolite compounds in Crescentia cujete L. has medicinal properties for diseases. Purpose: this study was to determine the phenolic compound of Crescentia cujete L which has acted as an anti-osteoporosis with the approach of docking in silico. Extraction and partitioning of Crescentia cujete L fruit extracts in this study used n-hexane and toluene solvents with the aim of obtaining phenolic group compounds, knowing the toxicity value of partition results, obtaining the structure of phenolic compounds that have the potential to be anti-osteoporosis, and validating the ability of these phenolic compounds as anti-oxidants. As a result, n-hexane and toluene extracts are known to contain elevated levels of active and toxic compounds with LC50 values of 31.79 ppm and 93.49 ppm, respectively. The phytochemical test shows the presence of phenolic groups with maximum wavelengths at 302-320 nm. Benzene acetate, trans-cinnamic acid, and propanoic acid have the lowest affinity bonds with receptor 2F8A (Glutathione peroxidase); -4.21, -4.81, and -4.72 kcal/mol. Whereas at the 3KWZ receptor (Cathepsin K); -6.0, -5.4, and -5.0 kcal/mol. Conclusion: The phenolic compound of Crescentia cujete extract L can be used as an alternative treatment for osteoporosis by inhibiting the enzymes Glutathione peroxidase and Cathepsin K. A B S T R A K Osteoporosis adalah penyakit tulang metabolik yang ditandai dengan penurunan massa tulang, yang disebabkan oleh penurunan matriks tulang dan mineral disertai dengan kerusakan mikroarsitektur jaringan tulang. Faktor-faktor seperti produksi hormon, proses penuaan, dan pembentukan radikal bebas akibat stres oksidatif oleh Reactive Oxygen Species (ROS) dapat merangsang resorpsi tulang. Kandungan senyawa metabolit sekunder pada Crescentia cujete L. memiliki khasiat obat untuk penyakit. Tujuan: penelitian ini untuk mengetahui senyawa fenolik Crescentia cujete L yang berperan sebagai anti osteoporosis dengan pendekatan docking in silico. Ekstraksi dan partisi ekstrak buah Crescentia cujete L pada penelitian ini menggunakan pelarut n-heksana dan toluena dengan tujuan untuk memperoleh senyawa golongan fenolik, mengetahui nilai toksisitas hasil partisi, memperoleh struktur senyawa fenolik yang berpotensi sebagai anti osteoporosis, dan memvalidasi kemampuan senyawa fenolik ini sebagai anti- oksidan. Hasilnya, ekstrak n-heksana dan toluena diketahui mengandung senyawa aktif dan toksik yang Teknika: Jurnal Sains dan Teknologi is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Toxicity and Polecular Gocking Dpproach Shenolic Hxtract of Crescentia cujete L against the enzymes Jlutathione peroxidase and Fathepsin K