~ 28 ~ Journal of Pharmacognosy and Phytochemistry 2017; 6(6): 28-30 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2017; 6(6): 28-30 Received: 19-09-2017 Accepted: 21-10-2017 Amit Sarker Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Nurul Amin Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Israt Jahan Shimu Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Md. Parvez Akhter Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Md. Ashickul Alam Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Md. Mizanur Rahman Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Habiba Sultana Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Correspondence Amit Sarker Department of Pharmacy, Primeasia University, Star Tower, 12 Kemal Ataturk Avenue, Banani, Dhaka, Bangladesh Antimicrobial activity of methanolic extract of langra mango pulp Amit Sarker, Nurul Amin, Israt Jahan Shimu, Md. Parvez Akhter, Md. Ashickul Alam, Md. Mizanur Rahman and Habiba Sultana Abstract Mango is antioxidant rich one of the most popular fruits in the world. Mangoes (Mangifera indica) belong to genus Mangifera under the family of Anacardiaceae. In Bangladesh, Mango is called as the “King of fruit” over a large area, but locally known as “aam”. Mango contains different types of chemicals and most of them are bioactive compounds such as polyphenols, vitamin C, carotenoids, anthocyanin, and flavonoids. The present study was to extract the chemicals from Langra mango as well as investigated the antimicrobial properties by using various in vitro methods. The mature fruit was extracted with methanol. Chemicals from mango pulps were extracted by using Rotary evaporator. Phytochemical tests were performed to investigate the possible phytochemicals present in the mango pulp extract. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were performed by disc diffusion and broth dilution assay against five (5) pathogenic bacteria. The antimicrobial activity exerted by the extract was compared with standard and results showed that extract contained significant antimicrobial activity. The order of zone of inhibition was Staphylococcus aureus (17mm) > Bacillus subtilis (16 mm) > Escherichia coli (14 mm) > Salmonella typhi (14 mm) > Klebsiella pneumoniae (12 mm). The MIC values were in the range 0.6 mg/ml to 1.25 mg/ml for extract of Langra mango pulp. The lowest MIC was found for Escherichia coli (0.6 mg/ml). So the extract (methanolic) of langra mango pulp has potent antimicrobial properties. Keywords: Langra mango pulp, Antimicrobial activity, Minimum Inhibitory Concentration (MIC), Pathogenic bacteria Introduction Medicinal plants are potential sources of drugs and of therapeutic importance because these plants have abundant secondary metabolites. This is why using of plant extracts as therapeutic agents is increasing day by day from ancient time [1, 2] . Because of natural origin and fewer side effects, Herbal medicine and these drugs are gaining acceptability and popularity day by day [3] . Beside the source of micronutrients, vitamins and other phytochemicals, Mango fruits are also provide energy, dietary fibres, carbohydrates, proteins, fats and phenolic compounds [4] . All components are vital not only for normal human growth but also for development and health [5] . Both unripe and ripe fruits have verity of ethnomedicinal use and effective against different diseases. The unripe fruits are acidic, digestive and carminative and useful against dysentery ophthalmia, eruptions, urethrorrhoea and vaginopathy. The ripe fruits are used against anorexia, dyspepsia, cardiopathy, haemoptysis, haemorrhages from uterus, lungs and intestine, emaciation, and anemia because they are refrigerant, sweet, emollient, laxative, cardiotonic, haemostatic, aphrodisiac, and tonic. Mango extract contain gallotannin and mangiferin. Presenting of these chemicals, extracts shows antibacterial activity against both Gram-positive and Gram-negative bacteria [6, 7, 8, 9] . Mangiferin (Mangiferin is a xanthonoid found in mangoes) is able to interfere with the assembly or functioning of microtubule filaments or cellular matrix components or inhibition of the telomerase and the gene. Another possible mechanism is the enhancement of the cellular apoptosis [10, 11, 12, 13, 14] . Hence the major objective of the present work was to evaluate the chemical composition, antibacterial effect activity of ripe mango pulp extract. Materials and Methods According to the investigational procedure the mature fruit mango were collected from Mohakhali Kacha bazar, Dhaka, Bangladesh in the month of May, 2016. Preparation of extract The pulp parts of the collected mango fruits were shade dried for seven days.