_____________________________________________________________________________________________________ ++ Ph.D; *Corresponding author: E-mail: mlihaaraka@gmail.com; Asian J. Biochem. Gen. Mol. Biol., vol. 14, no. 3, pp. 21-28, 2023 Asian Journal of Biochemistry, Genetics and Molecular Biology Volume 14, Issue 3, Page 21-28, 2023; Article no.AJBGMB.100924 ISSN: 2582-3698 Determination of Genetic Diversity of Tamarind (Tamarindus indica) Accessions in Eastern Region of Kenya Using Inter Simple Sequence Repeat (ISSR) Markers Mercy Liharaka Kidaha a++* , Cornelius Wainana Mbathi a++ and Fredah Rimberia Wanzala a++ a Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology, Kenya. Authors’ contributions This work was carried out in collaboration among all authors. All authors contributed to the study design and concept. Author MLK did the fieldwork, lab work, data analysis, interpretation and manuscript preparation. Author CWM did the data analysis and corrections of the paper. Author FRW did the field study and paper correction. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJBGMB/2023/v14i3316 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/100924 Received: 12/04/2023 Accepted: 16/06/2023 Published: 03/07/2023 ABSTRACT There is scanty information on tamarind’s genetic diversity in Easter Kenya. The objective of this study was to determine the genetic diversity of 64 tamarind accessions from Eastern Kenya using 12 Inter Simple Sequence Repeat (ISSR) markers. DNA was extracted from the young apical leaves using modified CTAB method and amplified using standard PCR. The data collected were Original Research Article Article