495 Journal of Scientific and Innovative Research 2014; 3(5): 495-498 Available online at: www.jsirjournal.com Research Article ISSN 2320-4818 JSIR 2014; 3(5): 495-498 © 2014, All rights reserved Received: 15-09-2014 Accepted: 05-10-2014 Rishikesh Kumar Dept. of Biomedical Informatics Centre, Rajendra Memorial Research Institute Medical Science, Patna 800007, India Ganesh Chandra Sahoo Dept. of Biomedical Informatics Centre, Rajendra Memorial Research Institute Medical Science, Patna 800007, India VNR Das Clinical Medicine, Rajendra Memorial Research Institute Medical Science, Patna 800007, India Krishna Pandey Clinical Medicine, Rajendra Memorial Research Institute Medical Science, Patna 800007, India Pradeep Das Dept. of Biomedical Informatics Centre, Rajendra Memorial Research Institute Medical Science, Patna 800007, India Correspondence: Ganesh Chandra Sahoo Dept. of Biomedical Informatics Centre, Rajendra Memorial Research Institute Medical Science, Patna 800007, India E-mail: ganeshhrmri@gmail.com Effects of core iron oxide nanoparticles on microbial control and bacteriostatic activity against Escherichia coli, Staphylococcus aureus and Mycobacterium smegmatis Rishikesh Kumar, Ganesh Chandra Sahoo*, VNR Das, Krishna Pandey, Pradeep Das Abstract The anti-bacterial activity of the Fe3/citric acid/cephalosporin nanoparticles (core) on Staphylococcus aureus (MTCC737), Mycobacterium smegmatis (MTCC994) and Escherichia coli (MTCC443) was tested using this system as an active compound carrier. We have describe the simple method to obtain iron nanoparticles with uniform size distribution by utilizing citric acid and as surface-capping agents the dimensions of Fe nanoparticles were in the 5-20 nm range and they were characterized by High Resolution Transmission Electron Microscopy (HRTEM).The particle size was tailored by tuning the concentration ratio of iron ions to carboxylic acid groups. Fe nanoparticles assisted by citric acid to analyze the interaction different type of cephalosporin against the these bacteria The antibacterial activity was observed in both, citric acid core nanoparticles/cephalosporin and core nanoparticles alone against these pathogen. Keywords: Nanofluid, Magnetic, Fe nanoparticles, Antibacterial, Cephalosporin, HRTEM. Introduction Magnetic nanoparticles have drawn much scientific interest by using magnetic nanoparticles for biological and medical purposes for a variety of studies. 1 Magnetic iron oxide based inorganic nanostructures materials have been synthesized and tested for various applications in medicine: as imaging agents, as heat mediators in hyperthermia treatments, in tissue repair, immunoassay, detoxification of biological fluids, cell separation, as magnetic guidance in drug delivery. The advantages of using these materials come from their magnetic properties, high surface area that provides higher sensitivity, better targeting and improvement of the colloidal stability of the nanostructures. 2 Important properties of the nanoparticles required for biomedical applications 3, 4 which are derived from a precise control of particle size, shape, dispersion and conditions that affect these properties. One area that is particularly promising is the use of magnetic nanoparticle systems for probing and manipulating biological systems. 5 Coating nanoparticles with natural or synthetic polymers or surfactants is a method that provides stability of the ferrofluid colloidal suspensions. Use of surfactants such as: citric acid, oleic acid, hexaldehyde or sodium carboxymethyl cellulose leads to highly dispersed and high quality nanoparticles with good biocompatibility and smaller particle size. 4 Coated nanoparticles are important for their lower toxicity due to the presence of the biocompatible coating, and also due to the lower adsorption sites for proteins, ions and other components in medium. 6 Usually iron oxides Fe 3 O 4 or γ- Fe 3 O 4 are synthesized through the co- precipitation of Fe 2+ and Fe 3+ aqueous salt solutions 5 , by addition of a base. Properties of nanoparticles such as size, shape and composition, are influenced by the type of salt, pH, ions ratio and ionic strength of the medium. 7 Some methods use magnetotactic bacteria (MTB) 8 that are able to internalize Fe and convert it into magnetic nanoparticles, in the form of either