469 Ngurah et al /J Applied Chem. Sci. 2018, 5(2): 469-472 Characterization of Cinnamadehyde Compound Isolated from Cinnamon Oil and Its Salmonella Typhy Antibacterial Activity Budiana I Gusti M. Ngurah 1 , Moses K. Tokan 2 , and Agus Saputra 3 1 Chemistry Study Program, Faculty of Teacher Training and Educational Sciences, Nusa Cendana University , 2 Biology Study Program, Faculty of Teacher Training and Educational Sciences, Nusa Cendana University, 3 Faculty of Veterinary, Nusa Cendana University, INDONESIA Article history: Received in revised form 15-05-2018 Accepted 25-08-2018 Available online September 28, 2018 Cite this article as: Budiana IGN, Moses KT, and Agus S. Characterization of Cinnamadehyde Compound Isolated from Cinnamon Oil and Its Salmonella Typhy Antibacterial Activity. J Applied Chem. Sci. 2018, 5(2): 469-472 DOI: https://dx.doi.org/10.22341/jacs.on.00502p469 p-ISSN: 2089-6328, e-ISSN: 2580-1953 © 2018 JACSOnline GP. All right served ABSTRACTS Today many infectious diseases are common. All of the diseases are caused by agents such as viruses or bacteria which are pathogenic and Salmonella typhi is one of the pathogenic bacteria. In addition, many antibiotics are not able to work properly because of the resistance of bacteria against the exciting antibiotics. Therefore, research to discover the new anti-bacterial compounds derived from natural materials is important to do. This research was conducted in several stages, that involved: (1) Isolation of cinnamaldehyde from cinnamon oil by two methods: thin layer chromatography using n-hexane: ethanol eluent at a ratio of 5 : 95 and bisulfate additional, (2) Identification of cinnamaldehyde by UV-Vis Spectrophotometer and GC-MS to define its purity, (3) The various concentrations of the cinnamaldehyde extracts were incubated with Salmonella typhi bacteria to evaluate the antibacterial activities of the extracts, (4) The growth of the tested bacteria was observed using a colony counter to see the diameter of the resistance which was caused by the test solution. Analysis result of Gas Chromatography – Mass Spectrophotometer showed that cinnamon oil (Cassia oil) contains cinnamaldehyde as the main component of 88.33 %. The isolation of cinnamaldehyde by bisulfate addition method obtained cinnamaldehyde as yellow fluid with a yield of 86.79 %. The antibacterial activity test indicated that cinnamaldehyde had the potential as an antibacterialagainst the Salmonella typhi which was known from its inhibition zone. At the concentration of 100 ppm, cinnamaldehyde solution showed an inhibitory diameter of 15.4 mm and amoxicillin antibiotic showed of 16.6 mm. Keywords: activity, antibacterial, cinnamaldehyde, isolation, characterization, inbihitory zone * Corresponding author: budianajelantik@gmail.com 1. Introduction An antibiotic is given for the treatment of diseases or infections caused by bacteria. Not only bacteria, target organisms of antibacterial also include fungi and other parasites (Nordqvist, 2009). Research on antibiotics continues to evolve over time and get the more attention of the researchers. This is related to the role of antibiotics in reducing death rates caused by infectious diseases. Some dangerous diseases caused by bacteria are tuberculosis, pneumonia, meningitis, typhoid, cholera, diarrhea, tetanus and others. Research on discovery of new antibiotic have been succeed to develop some effective antibiotic to against various types of bacteria but in the other side there are also strains of bacteria that are resistant to exiting antibiotics (Suwandi, 1992). The emergence of this resistance caused by bacteria can adapt to the presence of antibiotics in clinical concentration and also can be caused by the wrong usage of antibiotic by patient. Various studies discovered that 40-62 % caused by usage of antibiotic for the diseases that not require antibiotics (Jumina, 2015). Some antibiotics that have been resistant as reported (Rao 2012, 2015) are chloramphenicol (P.aeruginosa, K. pneumoniae, E. coli, S. typhimurium, V. cholerae), macrolides (Streptococcus pneumoniae, Enterococcus sps, Bacteroides sps, Pseudomonas sps and Enterobacteriaceae), tetracyclines (S. aureus, E. coli, A. baumannii, S. typhimurium), aminoglycosides (E. coli, P. aeruginosa, A. baumannii) and also beta-lactams (H. influenzae, P. aeruginosa, A. baumannii). In addition, there was increasing of 440,000 new cases due to multidrug-resistant tuberculosis (MDR-TB) each year which causes at least 150,000 cases of death each year. Indonesia ranked eighth out of 27 high MDR load countries (WHO, 2009). There is two ways to develop new antibiotics that are: 1) Isolation of the active compounds in medical plants that traditionally used to treat diseases caused by bacterial and 2) synthesized the groups of compounds that have been known to have antibiotic activity. Economically, the first way is advantageous if the antibacterial content in plans or microorganismis present in large quantities. However, if the The JACSOnline Group Publisher publishes the work of Jacson-Journal of Applied Chemical Science eISSN: 2580-1953/pISSN: 2089-6328 under the licensing of a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Authors retain the copyright to their work. Users may read, copy, and distribute the work in any medium provided the authors and the journal are appropriately credited. The users may not use the material for commercial purposes.