21 Research Paper Nanobiotechnology Role of phytochemicals on biosynthesis of silver nanoparticles from plant extracts and their concentration dependent toxicity impacts on Drosophila melanogaster Shruti Tyagi Women Scientist-A (WOS-A, DST), Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut, Uttar Pradesh, India A R T I C L E I N F O A B S T R A C T Received 15 May 2016 Revised 26 July 2016 Accepted 9 August 2016 We investigated the effects of phytochemical, ascorbic acid and total phenolic content on reduction of silver ions from plant materials and the toxicity of these silver ions on Drosophila melanogaster as an in vivo model system. In the presence of ascorbic acid concentration 38.88µg/gm and 35.5088µg/gm and the total phenolic contents concentration 97.89µg/gm and 26.99µg/gm in Chenopodium and Marigold extracts respectively, the multiple plasmon peaks were observed at the range of 360-426nm with absorbance of 0.780- 0.925cm -1 in Chenopodium extract with absorbance 0.392-0.569 cm -1 in marigold extract. The appearance of multiple surface plasmon peaks indicated the spherical shape of silver nanoparticles (primary characterization). Obtained multiple plasmon resonances at 360- 426nm and 310-450nm in Chenopodium and Marigold extracts, respectively may be indication of maximum reduction of Ag + and this enhancement of maximum reduction of Ag + due to the presence of ascorbic acid and total phenolic content higher concentrations. On the other hand, the quantitative measurement of AgNPs toxicity on Drosophila melanogaster a significant concentration-dependent decrease in survival rate, duration of development and melanization defects were observed for flies fed with AgNPs as compared to control Drosophila flies. K E Y W O R D S Drosophila melanogaster Nanotoxicity Silver nanoparticles Ascorbic acid Total phenolic contents Pigmentation *Corresponding author: ShrutiTyagi E-mail:stgenetics@gmail.com Tel: +919045096731 2016 Biological Innovations Research & Development Society