Anticancer Activity of Fibraurea Tinctoria with DLD1 Celline Cytotoxicity Assay Riski Sulistiarini 1,2* , Andreanus A. Soemardji 1 , Elfahmi 1 , Maria Immaculata Iwo 1 , Danang Waluyo 3 , Dian Japany Puspitasari 3 1 School of Pharmacy, Bandung Institute of Technology, Jl. Ganesha 10 Bandung, Indonesia, 40132 2 Pharmacy Faculty of Mulawarman University, Jl. Gn. Kelua, Samarinda, East Borneo, Indonesia, 75243 3 Biotech Center BPPT, Building 630 PUSPIPTEK Area, Setu, Tangerang Selatan, Banten 15314 Key Words: Anticancer, DLD1 Cel line, Cytotoxicity Assay Abstract: This study aims to determine the cytotoxic effects of Fibraurea tinctorial plants on DLD1 colon cancer cell lines in vitro and determine the IC50 values of these plants. The test begins with cell-line culture in the DMEM medium. Cultures that were ready with an estimated concentration in fresh media of 2.5x 104cells / 200uL were exposed to 0.4uL test material in 0.1% DMSO with concentrations starting at 6.25 ppm; 12.5 ppm; 25 ppm; 50 ppm; 100 ppm; 200ppm; 400ppm; 800ppm; and 1600ppm which was then incubated for 48 hours. The results are from methanol extract, hexane fraction, ethyl acetate fraction and HA and HB subfraction from Fibraurea tinctorial plants, only HB subfaction which shows inhibitory activity against DLD1 colon cancer cell line with 28% at 1600 ppm concentration.   1 INTRODUCTION The International Agency for Research on Cancer estimates the mortality and prevalence of cancer for 184 countries in the world at 14.1 million new cancer cases, with 8.2 million cancer deaths, and 32.6 million people living with cancer (within five years of diagnosis ) in 2012. By 2030, it is projected that there will be 26 million new cancer cases and 17 million cancer deaths per year (Solowey et al. 2014; Thun et al. 2010). Colorectal cancer is a disease that significantly attacks millions of people every year worldwide and is considered cancer with the third most common occurrence rate in men and number two in women. In the western world, with 655,000 deaths per year, colorectal cancer ranks third in cancer-causing deaths (Granlund et al. 2011; Selek et al. 2018). Natural products have played a major role in cancer chemotherapy. Anti-cancer drugs were introduced into therapy in Western countries for around 70 years, around 49% were either obtained from direct organisms or derived from natural material products (Gurnani et al. 2014). Actinomycin D, several anthracycline D derivatives (including daunorubicin, doxorubicin, epirubicin, idarubicin, and valrubicin), bleomycin, valcicin, cyclic and valrubicin derived from microbes that have been tested for cancer chemotherapy (Cragg, Grothaus, and Newman 2009) (Kinghorn et al. 2009). At present 4 ingredients that become anti-cancer drugs derived from plants are used clinically in the US and Europe, namely vinca (Catharanthus) bisindole alkaloids (vinblastin, vincristine, vinorelbine, vinflunine), semi-synthetic epipodophyllotoxins (etoposide, teniposide, and etoposide phosphate) ), taxel (paclitaxel and paclitaxel albumin-stable nanoparticle formulations, docetaxel, cabazitaxel), and campotekin derivatives (irinotecan and topotecan) (Kinghorn et al. 2016). In Indonesia itself, the use of medicines from natural ingredients is a culture and hereditary habits. Kayu kuning is a plant that is used as an anti-cancer therapy and also in conditions of digestive tract infections. One type of yellow wood is tinctorial Fibraurea (Wahyudi, Ratnadewi, and Siswoyo 2016). This plant was reported to have inhibitory activity against MCF-7 cell line from chloroform extract with IC50 value as significant as 11.2 ppm, although methanol and water extracts did not show significant activity to inhibit cell line extracted from breast cancer (Keawpradub and Dej-adisai 2005) Sulistiarini, R., Soemardji, A., Elfahmi, ., Iwo, M., Waluyo, D. and Puspitasari, D. Anticancer Activity of Fibraurea Tinctoria with DLD1 Celline Cytotoxicity Assay. DOI: 10.5220/0009126401710175 In Proceedings of the 2nd Health Science International Conference (HSIC 2019), pages 171-175 ISBN: 978-989-758-462-6 Copyright c  2020 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved 171