FOODSCITECH Food Science and Technology Journal 37 Matheus et al | Relative Mobility (Rf) Analysis… Relative Mobility (Rf) Analysis of Albumin Isolates from Snakehead Fish (Ophiocephalus striatus) Extracted at Different Temperatures and Times Matheus Nugroho 1 , Sugiono Sugiono 2 , Fadjar Kurnia Hartati 3 1 Department of Agriculture and Fisheries Technology, Faculty of Agriculture, Yudharta University, Pasuruan, East Java, Indonesia 2 Department of Fisheries Agribusiness, Faculty of Agriculture, Madura Islamic University, Pamekasan, East Java, Indonesia 3 Department of Food Technology, Faculty of Agriculture, Dr. Sutomo University, Surabaya, East Java, Indonesia Email: mtnugroho@gmail.com ABSTRACT Snakehead fish was a fish that has a high albumin content. Snakehead fish was used in the health sector known as medicinal freshwater fish, to accelerate the process of healing wounds after surgery and childbirth. The purpose of this study was to analyze the relative mobility (Rf) of the snakehead fish (Ophiocephalus striatus) albumin isolates by steaming at different temperatures and times. The research method for analyze the relative mobility (Rf) of the snakehead fish (Ophiocephalus striatus) albumin isolates by using gel filtration column chromatography, was detected by the SDS-PAGE electrophoresis method. Electrophoresis was carried out at a constant voltage of 125 v/ slab. Gel staining was carried out with Commasie Brilliant Blue R-250 in methanol-acetic acid-water. A standard protein mixture consisting of: myosin (200.0 kDa), β-galactosidase (116.3 kDa), phosphorylase B (97.4 kDa), bovine serum albumin (66, 2 kDa), ovalbumin (45.0 kDa), carbonic anhydrose (31.0 kDa), trypsin inhibitor (21.5 kDa), lysozyme (14.4 kDa) and aprotinim (6.5 kDa). The data analysis of this research was descriptive analysis to see the photos of the electrophoresis results. Meanwhile, for the analysis, the measurement of albumin isolation by gel filtration Sephadex G-75 was carried out with a divided plot design. The results showed that SDS-PAGE electrophoresis with the most complex amount of protein was albumin isolate, the effect of the steaming temperature was 40 o C for 30 minutes, located in 5 ml of the 1st fraction, 5 ml of the 2nd fraction and 5 ml of the 3rd fraction, with values of relative mobility (Rf) 0.0833 to 0.6944. Key words: snakehead fish; albumin isolate, relative mobility INTRODUCTION Albumin was the highest plasma protein in about 60% and has various functions that are very important for health, namely the formation of new cell tissue, accelerating the recovery of damaged body tissue and maintaining fluid balance in blood vessels with fluid in the interstitial cavity within the limits normal limit, albumin levels in the blood 3.5-5 g/dl (Fulks et al., 2010). One way to meet the needs of albumin in the body is by administering human serum albumin (HSA) (Kusumaningrum et al., 2014; Firlianty et al., 2014). Snakehead fish was a fish that has a high albumin content. Snakehead fish was used in the health sector known as medicinal freshwater fish, to accelerate the process of healing wounds after surgery and childbirth (Rahayu et al., 2016). Research in the nutrition installation and the surgical section of the Dr. Saiful Anwar Malang Hospital on postoperative patients with low albumin levels (1.8 g/dl). With the treatment of 2 kg of cooked snakehead fish per day, it has increased the patient's blood albumin levels to normal (3.5-5.5 g/dl) (Putri & Agustina, 2017). Snakehead fish has very beneficial biomedical benefits, such as anti- inflammatory, anti-microorganisms, anti-pain nociception and anti-cancer properties (He et al., 2017). Susilowati et al. (2015) in their research states that snakehead fish extract was a prospective alternative as raw material for nutraceutical products. Prastari et al. (2017) reported that snakehead fish protein hydrolyzate has antihyperglycemic potential. Other benefits of snakehead fish albumin, including maintaining intravascular oncotics (colloid