Annals of Clinical and Analytical Medicine 1237 Annals of Clinical and Analytical Medicine Original Research Abdullah Özer 1 , Ayşegül Küçük 2 , Yiğit Kılıç 3 , Serap Gültekin 4 , Hayriye Tatlı Doğan 5 , Aydan Kılıçarslan 5 , Hüseyin Demirtaş 1 , Barış Mardin 1 , Dilek Erer 1 Tolga Tatar 1 , Mustafa Arslan 6 1 Department of Cardiovascular Surgery, Faculty of Medicine, Gazi University, Ankara 2 Department of Physiology, Faculty of Medicine, Kütahya Health Science University, Kütahya 3 Department of Pediatric Cardiovascular Surgery, Dr Siyami Ersek Cardiovascular and Thoracic Surgery Training and Research Hospital, Istanbul 4 Department of Radiology, Faculty of Medicine, Gazi University, Ankara 5 Department of Pathology, Faculty of Medicine, Yıldırım Beyazıt University, Ankara 6 Department of Anesthesia and Reanimation, Faculty of Medicine, Gazi University, Ankara, Turkey Erdosteine on ischemia-reperfusion injury The efect of erdosteine on ischemia-reperfusion injury in skeletal muscle of rats DOI: 10.4328/ACAM.21268 Received: 2022-06-13 Accepted: 2022-07-13 Published Online: 2022-07-16 Printed: 2022-11-01 Ann Clin Anal Med 2022;13(11):1237-1240 Corresponding Author: Mustafa Arslan, Department of Anesthesia and Reanimation, Faculty of Medicine, Gazi University, 06510, Ankara, Turkey. E-mail: mustarslan@gmail.com P: +90 312 202 67 39 / + 90 533 422 85 77 Corresponding Author ORCID ID: https://orcid.org/0000-0003-4882-5063 Abstract Aim: As a mucolytic agent, Erdosteine has two sulfhydryl groups. These groups function as free radical scavengers, which has protective efects against ischemia- reperfusion (IR) injury. In this study, we aimed to observe the efects of erdosteine on skeletal muscle tissue in a rat lower extremity IR injury model. Material and Methods: A total of 18 Wistar albino rats were separated into 3 groups (n: 6); Control group (C), IR group (IR) and IR group with erdosteine (IR-E). Erdosteine administered intraperitoneally (150 mg.kg-1). Rat tissues were taken for histopathological and immunohistochemical evaluations afer 2 hours of ischemia and 2 hours of reperfusion period. Results: Blood fow measurements were signifcantly higher in the IR-E group than in the IR group. In the IR group, endothelial caspase 3 and 8 enzyme expression was signifcantly higher than C and IR-E groups. Muscle caspase 3 enzyme expression was higher in the IR group than C and IR-E groups. IR and IR-E groups showed increased infammation, vascular congestion and myositis injury levels than the C group. Also, infammation, vascular congestion and myositis injury showed a signifcant decrease in the IR-E group than IR group. Discussion: As a result, erdosteine has a protective efect against skeletal tissue damage resulting from IR injury in rats. The results show the possibility of clinical administration of erdosteine for I/R injury of skeletal muscle tissue. Keywords Skeletal Muscle, Ischemia-Reperfusion, Erdosteine, Caspase 3, Blood Flow, Rat