Polyaminocyclodextrin nanosponges: synthesis, characterization and pH-responsive sequestration abilities Marco Russo, a Maria Luisa Saladino, ab Delia Chillura Martino, ab Paolo Lo Meo * a and Renato Noto a New pH-responsive nanosponges were obtained by reacting four dierent polyaminocyclodextrins with heptakis-(6-bromo)-(6-deoxy)-b-cyclodextrin. The materials obtained were characterized by various techniques (FT-IR, potentiometric titration, dierential scanning calorimetry (DSC), porosimetry (BET), 13 C { 1 H} CP-MAS NMR). Their adsorption abilities at dierent pH values were veried towards a suitable set of model guests, and seem mainly controlled by electrostatic interactions, as a function of the protonation/ charge status of the polymer matrix. By contrast, data positively point out a lesser importance assumed by the induced-teect, important in aecting the formation of hostguest complexes in solution. The frequency-switched Lee-Goldburg (FSLG) heteronuclear correlation solid-state NMR technique was exploited in order to assess the possible location of the guests within the polymer matrix. Introduction Nanosponges (NSs) are a class of hyper-reticulated polymeric material, 1 which have attracted increasing interest during the past years, due to their adsorption or controlled release abilities towards various organic molecules, such as drugs, 1,2 conserva- tion agents 3 and pollutants. 4 These materials can be obtained by polymerizing or co-polymerizing macrocyclic species able to act as supramolecular hosts. Great attention has been devoted in particular to cyclodextrin-based NSs, which are usually obtained by treating native b-cyclodextrin (bCD) with double electro- philes such as epichlorohydrin, 5 carbonic acid derivatives 6 or bis-isocyanates. 7 The reaction, of course, exploits the nucleo- philic reactivity of the hydroxyl groups (both primary and secondary) on the bCD scaold, whereas the electrophile resi- dues function as linkers between the bCD subunits. Very recently, we have succeeded in exploiting a dierent approach, 8 i.e. a CuAAC reaction 9 between heptakis-(6-azido)-(6-deoxy)-bCD and a tetrakis-propargyloxy-calix[4]arene derivative. This procedure aords mixed co-polymers, having dierent average compositions depending on the combination ratio of the reac- tants. The potential use of these materials takes advantage of the somehow complementary binding abilities possessed by the two diverse macrocyclic hosts, and we veried that their adsorption abilities largely vary as a function of their actual composition. An interesting aspect related to the latter issue is the potential tunability of the co-polymers obtained, which can be hardly achieved for NSs based on bCD only. Sensitivity to external stimuli is an appealing goal in materials science. 10 In particular, systems able to change their properties in response to pH variations constitute an expanding area of research. 11 Just to cite a few examples, pH-tunable nanochannels for selective transmembrane ion transport have been built by means of polyelectrolyte brushes 12 or functionalized carbon nanotubes. 13 Molecular shuttles triggered by pH variations can mimic molecular muscles. 14 Reversible pH-dependent emulsifying action has been reported for chitosan. 15 Mesoporous silica containers equipped with supramolecular nanovalves have been used for drug delivery into acid generating cells. 16 In this context, the synthesis of pH-responsive NSs may constitute a very interesting task, in particular for the construction of drug carrier/delivery systems or pollutant removal devices. In the present work we report on the preparation, charac- terization, and sequestering abilities of a new class of NS materials, namely aminocyclodextrin nanosponges (ACN), Fig. 1 Structures of polyamines A1A4. a Universit` a degli Studi di Palermo-Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, V.le delle Scienze, Parco dOrleans, pad. 17, 90128 Palermo, Italy. E-mail: paolo.lomeo@unipa.it b CGA-ATeN Center Universit` a di Palermo, Via F. Marini, 14, 90128 Palermo, Italy Cite this: RSC Adv. , 2016, 6, 49941 Received 10th March 2016 Accepted 15th May 2016 DOI: 10.1039/c6ra06417e www.rsc.org/advances This journal is © The Royal Society of Chemistry 2016 RSC Adv. , 2016, 6, 4994149953 | 49941 RSC Advances PAPER