Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research __________________________________________________ ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(1):508-514 508 Effect of nonionic surfactant micellar (Triton X–100) on the spectrophotometric determination of Lead (II) with isonitriso-p- isopropyl acetophenone phenyl hydrazone Y.Sunandamma 1 , Som Shankar Dube 2 and Battula Sreenivasa Rao * 2 1 Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, AP. India 2 Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam, AP. India ______________________________________________________________________________ ABSTRACT The effect of nonionic surfactant micellar (Triton X–100) on the sensitive, simple and inexpensive, spectrophotometric method was developed for the determination of lead (II) with isonitriso p-isopropyl acetophenone phenyl hydrazone (HIPAPH). Lead was complexed with isonitriso p-isopropyl acetophenone phenyl hydrazone (HIPAPH) in presence of non ionic surfactant micellar (Triton X–100). Absorption spectrum of Pb- HIPAPH complex in the presence of non ionic surfactant 1 medium shows a peak at 390 nm. The molar absorptivity of the complex was 5.6734x10 4 dm 3 .mole -1 .cm -1 and Sandell’s sensitivity was 2.2367x10 -3 mg.cm -2 . The system obeys Beer’s law in the concentration range of 0.25 to 15.0ppm. The composition of the complex (metal: ligand) was 1:2. The method was employed for the determination of lead in industrial effluents and ground water samples. Key words: Micellar, spectrophotometric method, lead, isonitriso p-isopropyl Acetophenone Phenyl Hydrazone. INTRODUCTION Lead is a relatively abundant metal in nature, occurring in lead minerals. In the atmosphere it is relatively more abundant then other heavy metals. It is one of the metals extensively used in lead storage batteries and as tetra ethyl lead in combustion fuels. The major biochemical effect of lead