_____________________________________________________________________________________________________ *Corresponding author: E-mail: tudararo.aherobo@fupre.edu.ng; J. Appl. Life Sci. Int., vol. 26, no. 3, pp. 30-40, 2023 Journal of Applied Life Sciences International Volume 26, Issue 3, Page 30-40, 2023; Article no.JALSI.101746 ISSN: 2394-1103 Comparative Study of Metagenomic Profile of Bacteria Strains Present in an Abandoned Artisanal Refinery Site, in Obi-Ayagha Community, Delta State, Nigeria Tudararo-Aherobo, Laurelta Esivweneta a* and Okorhi, Beatrice Folawe a a Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, P.M.B. 1221, Effurun, Delta State, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author TALE designed the study, wrote the protocol, and reviewed the draft manuscript. Author OBF managed the analyses of the study and wrote the first draft of the manuscript. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JALSI/2023/v26i3605 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/101746 Received: 12/04/2023 Accepted: 15/06/2023 Published: 26/06/2023 ABSTRACT Aim: The aim of the study is to identify and compare the bacteria strains present in the control and Hydrocarbon impacted soil samples in the abandoned artisanal refinery site located in Obi-Ayagha community, Delta State, using high-throughput sequencing of the 16S rRNA gene. Place and Duration of Study: The Hydrocarbon impacted soil was collected from the abandoned artisanal refinery site located in Obi-Ayagha community and analysed in the advanced research laboratory of the Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun between 2022 and 2023. Original Research Article