Open Access Research Article Bari et al., Pharm Anal Acta 2011, 2:2 DOI: 10.4172/2153-2435.1000120 Volume 2 • Issue 2 • 1000120 Pharm Anal Acta ISSN: 2153-2435 PAA, an open access journal Keywords: Tamsulosin hydrochloride (TMS); UV- Spectrophotometry; First order derivative Introduction Tamsulosin hydrochloride is a sulphonamide derivative used in the treatment of Benign Prostate Hyperplasia (BPH), synonymously known as elders disease.TMS is ofcial in Martindale–Te Extra Pharmacopoeia and Merck Index [1,2]. TMS, (R)-5-[2-[[2-(2-ethoxyphenoxy)ethyl] amino]propyl]-2-methoxy-benzenesulfonamide, is a subtype selective μ 1A and μ 1D adrenoceptor antagonist approved by the Food and Drug Administration (FDA), USA for treatment of BPH. It is a new type of highly selective α-1-adrenergic receptor antagonist for treatment of BPH. Compared to other α-antagonists, tamsulosin hydrochloride has greater specifcity for α-1 receptors in the human prostate and does not afect receptors on blood vessels. Tamsulosin hydrochloride exists in two enantiomeric forms but only R-isomer is the pharmaceutically active component. Literature survey reveals the chiral separation by electrophoresis [3,4] and HPLC methods, coupled with ESI-MS-MS are reported for the estimation of tamsulosin hydrochloride with its impurities in bulk and pharmaceutical formulations [5] as well as in biological fuids [6-11]. According to our knowledge no method has been reported for TMS estimation by spectroscopic method. Te present work deals with estimation of tamsulosin hydrochloride in bulk and pharmaceutical formulation by simple and derivative spectroscopy which is economical and intended for better reproducibility of product. Material and Methods Chemicals All reagents used were of analytical grade. Instrument and conditions SHIMADZU AUX – 120 (Weighing Balance), UV Shimadzu 2450 (PC Series), UV-visible double beam spectrophotometer, Sofware UV Probe 2.21, Matched quartz cells 1 cm, Wavelength range 190 -900 nm, Lamp: 50 w, Deuterium Lamp, Detector: Silicon Photodiode, Cell holder: 1 mm wide, 12 mm high Resolution: 1 nm. Results and Discussion Tamsulosin hydrochloride in methanol: water (20:80) solvent system showed absorbance maximum at 280 nm. In ‘frst order *Corresponding author: P. S. Jain, R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur-425 405, Dhule (M.S.), Tel: +91 2563 255189; E-mail: pritash79@yahoo.com Received August 30, 2010; Published April 08, 2011 Citation: Bari SB, Bakshi AR, Jain PS, Surana SJ (2011) Application of UV- Spectroscopy and First Order Derivative Method for Determination of Tamsulosin Hydrochloride in Bulk and Tablets. Pharm Anal Acta 2:120. doi:10.4172/2153-2435.1000120 Copyright: © 2011 Bari SB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Two simple, rapid, sensitive and accurate UV-Spectrophotometry and First order derivative methods have been developed for estimation of tamsulosin hydrochloride in bulk and tablets. Methanol: water (2:8) was used as a solvent. In UV spectrophotometric method absorbance of samples are recorded at 280 nm. In frst order derivative method the amplitude of trough was recorded at 298 nm. Tamsulosin follows linearity in the concentration range of 10-90 μg/ml. Assay results were in good agreement with label claim. These method were validated statistically and recovery studies. Application of UV-Spectroscopy and First Order Derivative Method for Determination of Tamsulosin Hydrochloride in Bulk and Tablets S. B. Bari, A. R. Bakshi, P. S. Jain*, S. J. Surana R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur-425 405, Dhule (M.S.) Parameter At 280 nm At 298nm Linearity equation ( Y= a+ bc ) Y=0.0107X+0.002 0.0046X+ 0.0027 Range(µg/mL) 10.00-90.00 10.00 - 90.00 Correlation Coeffcient(r 2 ) 0.9993 0.9977 Table 1: Linear regression data for the calibration curves. (a) (b) Figure 1: Calibration curve for TMS at 280 nm (a) 298 nm (b). P h a r m a c e u t i c a A n a l y t i c a A c t a ISSN: 2153-2435 Pharmaceutica Analytica Acta