Journal of Applied Veterinary Science and Technology: 04(1).2023.24-29   http://e-journal.unair.ac.id/javest Occurrence of  Resistant to Some Selected Antibiotics Isolated from Farmed  Research Reports Resistensi  yang Diisolasi dari  yang Dibudidayakan terhadap Beberapa Jenis Antibiotik *1 Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin-Nigeria 2 Veterinary Teaching Hospital, Ahmadu Bello University, Zaria-Nigeria 3 Department of Zoology, Ahmadu Bello University, Zaria-Nigeria 4 Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin-Nigeria 5 Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ilorin, Ilorin-Nigeria Debora Arimie Adah 1* , Lawal Sa’idu 2 , Sonnie Joshua Oniye 3 , Moshood Abiola Raji 4 , Adakola Sylvanus Adah 5 ABSTRACT ARTICLE INFO Received: 28 January 2023 Revised: 19 February 2023 Accepted: 15 Maret 2023 Online: 30 April 2023 Journal of Applied Veterinary Science and Technology, p-ISSN: 2716-1188; e-ISSN: 2716-117X ©2023. Author(s). Open Acces Under Creative Commons Attribution-Share A Like 4.0 International License (CC-BY-SA)  One of the major causes of disease in Clarias gariepinus is Aeromonas spp. which has been linked to significant economic losses and poses a risk to public health.  The prevalence and hetero- geneity of Aeromonas species isolated from Clarias gariepinus cultured in concrete tanks, as well as the antibiogram and multiple antibiotic resistance index, were examined.  In this study, using culture, biochemical characterization, and Microbact 24E kit, Aeromonas spp. isolates were confirmed. The Kirby Bauer disc diffusion method assessed the antibiotic susceptibility to 10 different antibiotics.  Four different species of Aeromonas were identified, with an overall prevalence of 55 (28.6%). Aeromonas hydrophila had the highest prevalence of 35 (18.6%), followed by 10 (5.2%) for Aeromonas caviae, then 5 (2.6 %) for Aeromonas veronii biovar. sobria, and Aeromonas veronii biovar. veronii, respectively. The Aeromonas species exhibited high resistance to amoxicillin, ampicillin, colistin sulphate, oxytetracycline, trimethoprim/ sulphamethoxazole, and penicillin, with varied patterns of resistance, and the multiple antibiotic resistance (MAR) index values ranged between 0.10 and 0.80.  Several antibiotic resistant Aeromonas species were linked to the widespread emergence of antimicrobial resistance. As a result, it is crucial to regulate the use of antibiotics in fish farms and to ensure that biosecurity and preven- tive management strategies are applied effectively. ABSTRAK  Salah satu penyebab utama penyakit pada Clarias gariepinus adalah Aeromonas spp. Yang dikaitkan dengan kerugian ekonomi yang besar dan menimbulkan risiko bagi kesehatan masyarakat.  Untuk mengetahui prevalensi dan heterogenitas spesies Aeromonas yang diisolasi dari Clarias gariepinus yang dikultur dalam tangki beton, serta memeriksa antibiogram dan indeks resistensi antibiotik ganda.  Dalam penelitian ini menggunakan kultur, karakterisasi biokimia, dan kit Microbact 24E, isolat spesies Aeromonas yang terkonfirmasi. Metode difusi cakram Kirby Bauer digunakan untuk menilai kerentanan antibiotik terhadap 10 antibiotik yang berbeda.  Empat spesies Aeromonas yang berbeda diidentifikasi, dengan prevalensi keseluruhan 55 (28,6%). Aeromonas hydrophila memiliki prevalensi terting- gi 35 (18,6%) diikuti oleh 10 (5,2%) untuk Aeromonas caviae, kemudian 5 (2,6%) untuk Aeromonas veronii biovar. sobria, dan Aeromonas veronii biovar. veronii. Spesies Aeromonas menunjukkan resistensi tinggi terhadap amoksisilin, ampisilin, colistin sulfat, oxytetracycline, trimetoprim / sulphamethoxazole, dan penisi- lin, dengan pola resistensi yang bervariasi, dan nilai indeks resistensi antibiotik ganda (MAR) berkisar antara 0,10 dan 0,80.  Beberapa spesies Aeromonas yang kebal antibiotik dikaitkan dengan meluasn- ya kemunculan resistensi antimikroba. Oleh karena itu, penting untuk mengatur penggunaan antibiotik di peternakan ikan dan untuk memastikan bahwa strategi biosekuriti dan manajemen pencegahan diterapkan secara efektif. Keywords: Aquatic zoonosis, Freshwater fish, Multidrug resistance, MAR Index Kata kunci: Akuatik Zoonosis, Ikan air tawar, Resistensi Antibiotik, MAR Index * Correspondence: Debora Arimie Adah E-mail: adah.ad@unilorin.edu.ng Cite This Article:        24 10.20473/javest.V4.I1.2023.24-29