182 Behrouzyar and Herischi Int. J. Biosci. 2014 RESEARCH PAPER OPEN ACCESS Seed priming with ethanol, methanol, zinc, manganese, and boron enhances the seed mineral concentration and water deficit stress tolerance in canola (Brassica napus L.) Ebrahim Khalilvand Behrouzyar * , Mojgan Herischi Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, Tabriz Branch, Islamic Azad University, Tabriz, Iran Key words: Mineral concentration, oil percentage, seed priming, seed yield. http://dx.doi.org/10.12692/ijb/4.11.182-186 Article published on June 05, 2014 Abstract For studying the effects of seed priming with ethanol, methanol, Zn, B and Mn on seed mineral concentration and water deficit stress tolerance in Canola (Brassica napus L.), 2 separate laboratory and greenhouse experiments were conducted. Laboratory experiment was performed as a Completely Randomized Design with 6 priming treatments: (Non-primed, 2% ethanol, 2% methanol and 0.5% each ZnSo4, H3BO3, and MnSO4.H2O solutions) in three replicates. Greenhouse experiment was conducted as a factorial experiment form by Completely Randomized Design with 4 levels of irrigation: (100%, 75%, 50%, and 25% FC) with 3 replications. The results of the laboratory experiments indicated that the effect of the studied treatments on the seed mineral concentration was significant. Besides, the results of greenhouse experiments showed a significant effect of the seed yield and oil percentage. The detailed results of the study proved that Zn, B, and Mn application through canola seed treatments could be used to increase this mineral concentration in seeds compared with the control. Furthermore, ethanol priming has more pronounced effect for seed yield and oil percentage. Based on the results, the seed yield and oil percentage showed an average 85% and 5.06 % increase due to ethanol seed priming in 100% FC, respectively. * Corresponding Author: Ebrahim Khalilvand Behrouzyar  e.khalilvand@iaut.ac.ir International Journal of Biosciences | IJB | ISSN: 2220-6655 (Print) 2222-5234 (Online) http://www.innspub.net Vol. 4, No. 11, p. 182-186, 2014